BMRB Entry 51304

Name: Backbone assignment for human Cdc20 (1-73)
Published: 2022-09-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51304

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment for human Cdc20 (1-73)

Deposition date:

2022-02-05

Original release date:

2022-09-09

Authors:

Fischer, Elyse; Yu, Conny; Hevler, Johannes; McLaughlin, Stephen; Tamara, Sem; Maslen, Sarah; Cowieson, Nathan; Heck, Albert; Freund, Stefan; Barford, David

Citation:

Citation: Fischer, Elyse; Yu, Conny; Hevler, Johannes; McLaughlin, Stephen; Maslen, Sarah; Heck, Albert; Freund, Stefan; Barford, David. "Juxtaposition of Bub1 and Cdc20 on phosphorylated Mad1 during catalytic mitotic checkpoint complex assembly" Nat. Commun. 13, 6381-6381 (2022).
PubMed: 36289199

Assembly members:

Assembly members:
entity_1, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSFDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAQFAFESDLHSLLQLDAPI PNAPPARWQRKAKEAAGPAP SPMRAANRSHSAGRTPGRTP GKSSSKVQTTPSK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	215
15N chemical shifts	72
1H chemical shifts	57

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cdc20(1-73)	1

Entities:

Entity 1, Cdc20(1-73) 73 residues - Formula weight is not available

1

MET

ALA

GLN

PHE

ALA

PHE

GLU

SER

ASP

LEU

2

HIS

SER

LEU

GLN

LEU

ASP

ALA

PRO

ILE

3

PRO

ASN

ALA

PRO

ALA

ARG

TRP

GLN

ARG

4

LYS

ALA

LYS

GLU

ALA

GLY

PRO

ALA

PRO

5

SER

PRO

MET

ARG

ALA

ASN

ARG

SER

HIS

6

SER

ALA

GLY

ARG

THR

PRO

GLY

ARG

THR

PRO

7

GLY

LYS

SER

LYS

VAL

GLN

THR

8

PRO

SER

LYS

Samples:

sample_1: Cdc20(1-73), [U-13C; U-15N; U-2H], 200 uM; HEPES 20 mM; sodium chloride 100 mM; TCEP 1 mM; sodium azide 0.02%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
2D 13C-detected CON	sample_1	isotropic	sample_conditions_1
3D 13C-detected CBCACON	sample_1	isotropic	sample_conditions_1
3D 13C-detected CBCANCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.1 - collection, processing

NMRFAM-SPARKY v1.47 - chemical shift assignment, data analysis, peak picking

MddNMR - processing

MARS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE II+ 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks