BMRB Entry 51287

Title:
S. cerevisiae SERF sidechain
Deposition date:
2022-01-22
Original release date:
2022-02-02
Authors:
Liu, Yicong
Citation:

Citation: Liu, Yicong; Wang, Chaozhe; Jin, Yangzhuoyue; Jiang, Guosheng; He, Lichun; Liu, Maili. "Backbone resonance assignments and dynamics of S. cerevisiae SERF"  Biomol. NMR Assignments 16, 187-190 (2022).
PubMed: 35713792

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts127
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S. cerevisiae SERF1

Entities:

Entity 1, S. cerevisiae SERF 68 residues - Formula weight is not available

1   METALAARGGLYASNGLNARGASPLEUALA
2   ARGGLNLYSASNLEULYSLYSGLNLYSASP
3   METALALYSASNGLNLYSLYSSERGLYASP
4   PROLYSLYSARGMETGLUSERASPALAGLU
5   ILELEUARGGLNLYSGLNALAALAALAASP
6   ALAARGARGGLUALAGLULYSLEUGLULYS
7   LEULYSALAGLULYSTHRARGARG

Samples:

sample_1: S. cerevisiae SERF, [U-100% 15N], 400 uM; sodium chloride 50 mM; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz