BMRB Entry 51287
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51287
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Citation: Liu, Yicong; Wang, Chaozhe; Jin, Yangzhuoyue; Jiang, Guosheng; He, Lichun; Liu, Maili. "Backbone resonance assignments and dynamics of S. cerevisiae SERF" Biomol. NMR Assignments 16, 187-190 (2022).
PubMed: 35713792
Assembly members:
entity_1, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a
Entity Sequences (FASTA):
entity_1: MARGNQRDLARQKNLKKQKD
MAKNQKKSGDPKKRMESDAE
ILRQKQAAADARREAEKLEK
LKAEKTRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 127 |
| 1H chemical shifts | 232 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | S. cerevisiae SERF | 1 |
Entities:
Entity 1, S. cerevisiae SERF 68 residues - Formula weight is not available
| 1 | MET | ALA | ARG | GLY | ASN | GLN | ARG | ASP | LEU | ALA | ||||
| 2 | ARG | GLN | LYS | ASN | LEU | LYS | LYS | GLN | LYS | ASP | ||||
| 3 | MET | ALA | LYS | ASN | GLN | LYS | LYS | SER | GLY | ASP | ||||
| 4 | PRO | LYS | LYS | ARG | MET | GLU | SER | ASP | ALA | GLU | ||||
| 5 | ILE | LEU | ARG | GLN | LYS | GLN | ALA | ALA | ALA | ASP | ||||
| 6 | ALA | ARG | ARG | GLU | ALA | GLU | LYS | LEU | GLU | LYS | ||||
| 7 | LEU | LYS | ALA | GLU | LYS | THR | ARG | ARG |
Samples:
sample_1: S. cerevisiae SERF, [U-100% 15N], 400 uM; sodium chloride 50 mM; potassium phosphate 20 mM
sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker Avance 850 MHz