BMRB Entry 51283

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for vRAGE(23-125)
Deposition date:
2022-01-18
Original release date:
2022-10-19
Authors:
Long, Houfang; zhang, shengnan; Zeng, Shuyi; Liu, Cong; Li, Dan
Citation:

Citation: Long, Houfang; Zhang, Shengnan; Zeng, Shuyi; Tong, Yilun; Liu, Jun; Liu, Cong; Li, Dan. "Interaction of RAGE with a-synuclein fibrils mediates inflammatory response of microglia"  Cell Rep. 40, 111401-111401 (2022).
PubMed: 36130498

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Data sets:
Data typeCount
13C chemical shifts166
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1vRAGE1

Entities:

Entity 1, vRAGE 104 residues - Formula weight is not available

The construct was made for the residues 23-125 of human RAGE, and refereed as vRAGE.

1   GLYALAGLNASNILETHRALAARGILEGLY
2   GLUPROLEUVALLEULYSCYSLYSGLYALA
3   PROLYSLYSPROPROGLNARGLEUGLUTRP
4   LYSLEUASNTHRGLYARGTHRGLUALATRP
5   LYSVALLEUSERPROGLNGLYGLYGLYPRO
6   TRPASPSERVALALAARGVALLEUPROASN
7   GLYSERLEUPHELEUPROALAVALGLYILE
8   GLNASPGLUGLYILEPHEARGCYSGLNALA
9   METASNARGASNGLYLYSGLUTHRLYSSER
10   ASNTYRARGVALARGVALTYRGLNILEPRO
11   GLYLYSPROGLU

Samples:

sample_1: vRAGE(23-125), [U-99% 13C; U-99% 15N], 440 uM; D2O, [U-100% 2H], 10 % v/v; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - data analysis

NMRDraw - chemical shift assignment

NMR spectrometers:

  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks