BMRB Entry 51283

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for vRAGE(23-125)
Published: 2022-10-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51283

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for vRAGE(23-125)

Deposition date:

2022-01-18

Original release date:

2022-10-19

Authors:

Long, Houfang; zhang, shengnan; Zeng, Shuyi; Liu, Cong; Li, Dan

Citation:

Citation: Long, Houfang; Zhang, Shengnan; Zeng, Shuyi; Tong, Yilun; Liu, Jun; Liu, Cong; Li, Dan. "Interaction of RAGE with a-synuclein fibrils mediates inflammatory response of microglia" Cell Rep. 40, 111401-111401 (2022).
PubMed: 36130498

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAQNITARIGEPLVLKCKGA PKKPPQRLEWKLNTGRTEAW KVLSPQGGGPWDSVARVLPN GSLFLPAVGIQDEGIFRCQA MNRNGKETKSNYRVRVYQIP GKPE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	166
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	vRAGE	1

Entities:

Entity 1, vRAGE 104 residues - Formula weight is not available

The construct was made for the residues 23-125 of human RAGE, and refereed as vRAGE.

1

GLY

ALA

GLN

ASN

ILE

THR

ALA

ARG

ILE

GLY

2

GLU

PRO

LEU

VAL

LEU

LYS

CYS

LYS

GLY

ALA

3

PRO

LYS

PRO

GLN

ARG

LEU

GLU

TRP

4

LYS

LEU

ASN

THR

GLY

ARG

THR

GLU

ALA

TRP

5

LYS

VAL

LEU

SER

PRO

GLN

GLY

PRO

6

TRP

ASP

SER

VAL

ALA

ARG

VAL

LEU

PRO

ASN

7

GLY

SER

LEU

PHE

LEU

PRO

ALA

VAL

GLY

ILE

8

GLN

ASP

GLU

GLY

ILE

PHE

ARG

CYS

GLN

ALA

9

MET

ASN

ARG

ASN

GLY

LYS

GLU

THR

LYS

SER

10

ASN

TYR

ARG

VAL

ARG

VAL

TYR

GLN

ILE

PRO

11

GLY

LYS

PRO

GLU

Samples:

sample_1: vRAGE(23-125), [U-99% 13C; U-99% 15N], 440 uM; D2O, [U-100% 2H], 10 % v/v; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - data analysis

NMRDraw - chemical shift assignment

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks