BMRB Entry 51282

Name: NMR Backbone Assignment of Nup358-Min
Published: 2022-01-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51282

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Backbone Assignment of Nup358-Min

Deposition date:

2022-01-14

Original release date:

2022-01-25

Authors:

Gibson, James; Wang, Chunyu; Zhao, Jing

Citation:

Citation: Gibson, James; Cui, Heying; Ali, M Yusuf; Zhao, Xiaoxin; Debler, Erik; Zhao, Jing; Trybus, Kathleen; Solmaz, Sozanne; Wang, Chunyu. "Coil-to-alpha-helix transition at the Nup358-BicD2 interface activates BicD2 for dynein recruitment" eLife 11, e74714-e74714 (2022).
PubMed: 35229716

Assembly members:

Assembly members:
entity_1, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6p1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSDIPLQTPHKLVDTGR AAKLIQRAEEMKSGLKDFKT FLTNDQTKVTEEENKGSGTG AAGASDTTIKPNPENTGPTL EWDNYDLREDALDDSVSS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	257
15N chemical shifts	78
1H chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nup358-Min	1

Entities:

Entity 1, Nup358-Min 98 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

ASP

ILE

PRO

LEU

GLN

2

THR

PRO

HIS

LYS

LEU

VAL

ASP

THR

GLY

ARG

3

ALA

LYS

LEU

ILE

GLN

ARG

ALA

GLU

4

MET

LYS

SER

GLY

LEU

LYS

ASP

PHE

LYS

THR

5

PHE

LEU

THR

ASN

ASP

GLN

THR

LYS

VAL

THR

6

GLU

ASN

LYS

GLY

SER

GLY

THR

GLY

7

ALA

GLY

ALA

SER

ASP

THR

ILE

LYS

8

PRO

ASN

PRO

GLU

ASN

THR

GLY

PRO

THR

LEU

9

GLU

TRP

ASP

ASN

TYR

ASP

LEU

ARG

GLU

ASP

10

ALA

LEU

ASP

SER

VAL

SER

Samples:

sample_1: Nup358-min, [U-99% 13C; U-99% 15N], 0.2 mM; HEPES 20 mM; TCEP 0.5 mM

sample_conditions_1: pH: 7.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

SMILE - Reconstruction of NUS

NMRFAM-SPARKY - data analysis

PINE - refinement

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks