Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51280
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Citation: MacKenzie, Duncan; Schaefer, Anna; Steckner, Julia; Leo, Christopher; Naser, Dalia; Artikis, Eforsini; Broom, Aron; Ko, Travis; Shah, Purnak; Ney, Mikaela; Tran, Elisa; Smith, Martin; Fuglestad, Brian; Wand, A Josh; Brooks, Charles; Meiering, Elizabeth. "A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein" Proc. Natl. Acad. Sci. U.S.A. 119, e2119686119-e2119686119 (2022).
PubMed: 35737838
Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: cellular slime molds Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
entity_1: MGNRAFKSHHGHFLSAEGEA
VKTHHGHHDHHTHFHVENHG
GKVALKTHCGKYLSIGDHKQ
VYLSHHLHGDHSLFHLEHHG
GKVSIKGHHHHYISADHHGH
VSTKEHHDHDTTFEEIII
Data type | Count |
15N chemical shifts | 86 |
1H chemical shifts | 86 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | WT non-myristoylated hisactophilin | 1 |
Entity 1, WT non-myristoylated hisactophilin 118 residues - Formula weight is not available
1 | MET | GLY | ASN | ARG | ALA | PHE | LYS | SER | HIS | HIS | ||||
2 | GLY | HIS | PHE | LEU | SER | ALA | GLU | GLY | GLU | ALA | ||||
3 | VAL | LYS | THR | HIS | HIS | GLY | HIS | HIS | ASP | HIS | ||||
4 | HIS | THR | HIS | PHE | HIS | VAL | GLU | ASN | HIS | GLY | ||||
5 | GLY | LYS | VAL | ALA | LEU | LYS | THR | HIS | CYS | GLY | ||||
6 | LYS | TYR | LEU | SER | ILE | GLY | ASP | HIS | LYS | GLN | ||||
7 | VAL | TYR | LEU | SER | HIS | HIS | LEU | HIS | GLY | ASP | ||||
8 | HIS | SER | LEU | PHE | HIS | LEU | GLU | HIS | HIS | GLY | ||||
9 | GLY | LYS | VAL | SER | ILE | LYS | GLY | HIS | HIS | HIS | ||||
10 | HIS | TYR | ILE | SER | ALA | ASP | HIS | HIS | GLY | HIS | ||||
11 | VAL | SER | THR | LYS | GLU | HIS | HIS | ASP | HIS | ASP | ||||
12 | THR | THR | PHE | GLU | GLU | ILE | ILE | ILE |
sample_1: MES 50 mM; EDTA 1 mM; DTT 1 mM; DSS 1 mM; WT non-myristoylated hisactophilin, [U-99% 15N], 28 mg/mL
sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 299.45 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
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