BMRB Entry 51273

Name: Chemical shift assignment of I85L non-myristoylated Hisactophilin at pH 7.7
Published: 2022-06-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51273

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignment of I85L non-myristoylated Hisactophilin at pH 7.7

Deposition date:

2022-01-13

Original release date:

2022-06-24

Authors:

MacKenzie, Duncan; Leo, Christopher; Ko, Travis; Shah, Purnak; Ney, Mikaela; Smith, Martin; Meiering, Elizabeth

Citation:

Citation: MacKenzie, Duncan; Schaefer, Anna; Steckner, Julia; Leo, Christopher; Naser, Dalia; Artikis, Eforsini; Broom, Aron; Ko, Travis; Shah, Purnak; Ney, Mikaela; Tran, Elisa; Smith, Martin; Fuglestad, Brian; Wand, A Josh; Brooks, Charles; Meiering, Elizabeth. "A fine balance of hydrophobic-electrostatic communication pathways in a pH-switching protein" Proc. Natl. Acad. Sci. U.S.A. 119, e2119686119-e2119686119 (2022).
PubMed: 35737838

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: cellular slime molds Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGNRAFKSHHGHFLSAEGEA VKTHHGHHDHHTHFHVENHG GKVALKTHCGKYLSIGDHKQ VYLSHHLHGDHSLFHLEHHG GKVSLKGHHHHYISADHHGH VSTKEHHDHDTTFEEIII

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	72
1H chemical shifts	72

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	I85L non-myristoylated hisactophilin	1

Entities:

Entity 1, I85L non-myristoylated hisactophilin 118 residues - Formula weight is not available

1

MET

GLY

ASN

ARG

ALA

PHE

LYS

SER

HIS

2

GLY

HIS

PHE

LEU

SER

ALA

GLU

GLY

GLU

ALA

3

VAL

LYS

THR

HIS

GLY

HIS

ASP

HIS

4

HIS

THR

HIS

PHE

HIS

VAL

GLU

ASN

HIS

GLY

5

GLY

LYS

VAL

ALA

LEU

LYS

THR

HIS

CYS

GLY

6

LYS

TYR

LEU

SER

ILE

GLY

ASP

HIS

LYS

GLN

7

VAL

TYR

LEU

SER

HIS

LEU

HIS

GLY

ASP

8

HIS

SER

LEU

PHE

HIS

LEU

GLU

HIS

GLY

9

GLY

LYS

VAL

SER

LEU

LYS

GLY

HIS

10

HIS

TYR

ILE

SER

ALA

ASP

HIS

GLY

HIS

11

VAL

SER

THR

LYS

GLU

HIS

ASP

HIS

ASP

12

THR

PHE

GLU

ILE

Samples:

sample_1: potassium phosphate 50 mM; EDTA 1 mM; DTT 1 mM; DSS 1 mM; WT myristoylated hisactophilin, [U-99% 15N], 28 mg/mL

sample_conditions_1: pH: 7.7; pressure: 1 atm; temperature: 298.95 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5 - chemical shift assignment, collection, processing

xwinnmr - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks