BMRB Entry 51262

Title:
histone H3 H4 N-tail in nucleosome
Deposition date:
2022-01-06
Original release date:
2022-02-07
Authors:
Furukawa, Ayako; Nishimura, Yoshihumi
Citation:

Citation: Furukawa, Ayako; Wakamori, Masatoshi; Arimura, Yasuhiro; Ohtomo, Hideaki; Tsunaka, Yasuo; Kurumizaka, Hitoshi; Umehara, Takashi; Nishimura, Yoshihumi. "Acetylated histone H4 tail enhances histone H3 tail acetylation by altering their mutual dynamics in the nucleosome"  Proc. Natl. Acad. Sci. U. S. A. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 112 residues, Formula weight is not available
entity_2, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts127
15N chemical shifts46
1H chemical shifts46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H31
2H42

Entities:

Entity 1, H3 112 residues - Formula weight is not available

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLULEU
7   LEUILEARGLYSLEUPROPHEGLNARGLEU
8   VALARGGLUILEALAGLNASPPHELYSTHR
9   ASPLEUARGPHEGLNSERALAALAILEHIS
10   ALALYSARGVALTHRILEMETPROLYSASP
11   ILEGLNLEUALAARGARGILEARGGLYGLU
12   ARGALA

Entity 2, H4 102 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSGLYLEU
2   GLYLYSGLYGLYALALYSARGHISARGLYS
3   VALLEUARGASPASNILEGLNGLYILETHR
4   LYSPROALAILEARGARGLEUALAARGARG
5   GLYGLYVALLYSARGILESERGLYLEUILE
6   TYRGLUGLUTHRARGGLYVALLEULYSVAL
7   PHELEUGLUASNVALILEARGASPALAVAL
8   THRTYRTHRGLUHISALALYSARGLYSTHR
9   VALTHRALAMETASPVALVALTYRALALEU
10   LYSARGGLNGLYARGTHRLEUTYRGLYPHE
11   GLYGLY

Samples:

sample_1: H3 histone tail, [U-13C; U-15N; U-2H], 70 uM; H4 histone tail, [U-100% 13C; U-100% 15N], 70 uM; DTT 2 mM

sample_2: H3 histone tail, [U-13C; U-15N; U-2H], 70 uM; H4 histone tail, [U-100% 13C; U-100% 15N], 70 uM; DTT 2 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6; temperature: 298 K

sample_conditions_2: ionic strength: 25 mM; pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3DHN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

MagRO-NMRView - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks