BMRB Entry 5126

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5126

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete 1H, 15N and 13C Assignment of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor (CNTFR)

Deposition date:

2001-08-28

Original release date:

2002-04-08

Authors:

Man, D.; Xia, Y.; Sze, K.; Smith, D.; He, W.; Ip, N.; Zhu, G.

Citation:

Citation: Man, D.; Xia, Y.; Sze, K.; Smith, D.; He, W.; Ip, N.; Zhu, G.. "Complete 1H, 15N and 13C Assignment of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor (CNTFR)" J. Biomol. NMR 22, 95-96 (2002).
PubMed: 11885987

Assembly members:

Assembly members:
Ciliary Neurotrophic Factor Receptor, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ciliary Neurotrophic Factor Receptor: GPLGSVKPDPPENVVARPVP SNPRRLEVTWQTPSTWPDPE SFPLKFFLRYRPLILDQWQH VELSNGTAHTITDAYAGKEY IIQVAAKDNEIGTWSDWSVA AHATPWTEE

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	730
13C chemical shifts	451
15N chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CNTFR subunit 1	1

Entities:

Entity 1, CNTFR subunit 1 109 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

VAL

LYS

PRO

ASP

PRO

2

PRO

GLU

ASN

VAL

ALA

ARG

PRO

VAL

PRO

3

SER

ASN

PRO

ARG

LEU

GLU

VAL

THR

TRP

4

GLN

THR

PRO

SER

THR

TRP

PRO

ASP

PRO

GLU

5

SER

PHE

PRO

LEU

LYS

PHE

LEU

ARG

TYR

6

ARG

PRO

LEU

ILE

LEU

ASP

GLN

TRP

GLN

HIS

7

VAL

GLU

LEU

SER

ASN

GLY

THR

ALA

HIS

THR

8

ILE

THR

ASP

ALA

TYR

ALA

GLY

LYS

GLU

TYR

9

ILE

GLN

VAL

ALA

LYS

ASP

ASN

GLU

10

ILE

GLY

THR

TRP

SER

ASP

TRP

SER

VAL

ALA

11

ALA

HIS

ALA

THR

PRO

TRP

THR

GLU

Samples:

sample_1: Ciliary Neurotrophic Factor Receptor, [U-99% 15N], 2 mM

sample_2: Ciliary Neurotrophic Factor Receptor, [U-99% 13C; U-99% 15N], 1.3 mM

Ex-cond_1: pH*: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HACAN	not available	not available	not available
HACA(CO)N	not available	not available	not available
HACACO	not available	not available	not available
HNCO	not available	not available	not available
3D 15N separated TOCSY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HCC-TOCSY-NNH	not available	not available	not available

Software:

nmrpipe v1.6 - processing, assignment

pipp - processing, assignment

sparky v2.9 - processing, assignment

NMR spectrometers:

Varian inova 750 MHz
Varian inova 500 MHz

PDB	1UC6
DBJ	BAE23207 BAG54504 BAI44631 BAI46662
GB	AAA35707 AAA91337 AAB25290 AAB25918 AAC25711
REF	NP_001003353 NP_001003929 NP_001129528 NP_001193940 NP_001244612
SP	O88507 P26992 Q08406 Q71DR4
TPG	DAA26659
AlphaFold	O88507 P26992 Q08406 Q71DR4