BMRB Entry 51259

Title:
Islet amyloid polypeptide (amylin) in solution pH 6.5 10degC
Deposition date:
2022-01-05
Original release date:
2022-01-20
Authors:
Torner, Ricarda; Kupreichyk, Tatsiana; Gremer, Lothar; Colas Debled, Elisa; Fenel, Dapna; Gans, Pierre; Willbold, Dieter; Schoehn, Guy; Hoyer, Wolfgang; Boisbouvier, Jerome
Citation:

Citation: Torner, Ricarda; Kupreichyk, Tatsiana; Gremer, Lothar; Debled, Elisa Colas; Fenel, Daphna; Schemmert, Sarah; Gans, Pierre; Willbold, Dieter; Schoehn, Guy; Hoyer, Wolfgang; Boisbouvier, Jerome. "Structural basis for the inhibition of IAPP fibril formation by the co-chaperonin prefoldin"  Nat. Commun. 13, 2363-2363 (2022).
PubMed: 35501361

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET302/NT-His

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:
Data typeCount
13C chemical shifts103
15N chemical shifts34
1H chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hIAPP1

Entities:

Entity 1, hIAPP 37 residues - Formula weight is not available

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Samples:

sample_1: human Islet amyloid polypeptide (hIAPP), [U-100% 13C; U-100% 15N], 80 uM; MES/NaOH 25 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks