BMRB Entry 51256

Title:
Inter-domain flexibility of human SRSF1 tandem RRMs allows flexibility in RNA binding
Deposition date:
2022-01-03
Original release date:
2023-02-16
Authors:
De Silva, Naiduwadura Ivon; Zhang, Jun
Citation:

Citation: De Silva, Naiduwadura Ivon Upekala; Fargason, Talia; Zhang, Zihan; Wang, Ting; Zhang, Jun. "Inter-domain Flexibility of Human Ser/Arg-Rich Splicing Factor 1 Allows Variable Spacer Length in Cognate RNA's Bipartite Motifs"  Biochemistry 61, 2922-2932 (2022).
PubMed: 36454680

Assembly members:

Assembly members:
entity_1, polymer, 196 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSMT3

Data sets:
Data typeCount
13C chemical shifts485
15N chemical shifts164
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein1
2RNA2

Entities:

Entity 1, Protein 196 residues - Formula weight is not available

1   METSERGLYGLYGLYVALILEARGGLYPRO
2   ALAGLYASNASNASPCYSARGILETYRVAL
3   GLYASNLEUPROPROASPILEARGTHRLYS
4   ASPILEGLUASPVALPHETYRLYSTYRGLY
5   ALAILEARGASPILEASPLEULYSASNARG
6   ARGGLYGLYPROPROPHEALAPHEVALGLU
7   PHEGLUASPPROARGASPALAGLUASPALA
8   VALTYRGLYARGASPGLYTYRASPTYRASP
9   GLYTYRARGLEUARGVALGLUPHEPROARG
10   SERGLYARGGLYTHRGLYARGGLYGLYGLY
11   GLYGLYGLYGLYGLYGLYALAPROARGGLY
12   ARGTYRGLYPROPROSERARGARGSERGLU
13   ASNARGVALVALVALSERGLYLEUPROPRO
14   SERGLYSERTRPGLNASPLEULYSASPHIS
15   METARGGLUALAGLYASPVALCYSTYRALA
16   ASPVALTYRARGASPGLYTHRGLYVALVAL
17   GLUPHEVALARGLYSGLUASPMETTHRTYR
18   ALAVALARGLYSLEUASPASNTHRLYSPHE
19   ARGSERHISGLUGLYGLUTHRALATYRILE
20   ARGVALLYSVALASPGLY

Entity 2, RNA 8 residues - Formula weight is not available

1   UCAGAGGA

Samples:

sample_1: SRSF1_1-196, [U-13C; U-15N; U-2H], 436 uM; Single stranded 8mer RNA 620 uM; MES 20 mM; Arg/Glu Acid 380 mM

sample_conditions_1: pH: 6.2; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III HD 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks