BMRB Entry 51254

Title:
Solution NMR backbone chemical shift assignment for the extended xRRM2 domain of human La-related protein 7
Deposition date:
2021-12-22
Original release date:
2022-03-28
Authors:
Yang, Yuan; Eichhorn, Catherine; Hadjian, Tanya; Feigon, Juli
Citation:

Citation: Yang, Yuan; Liu, Shiheng; Egloff, Sylvain; Eichhorn, Catherine; Hadjian, Tanya; Zhen, James; Kiss, Tamas; Zhou, Z. Hong; Feigon, Juli. "Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP"  Mol. Cell 82, 1724-1736 (2022).
PubMed: 35320752

Assembly members:

Assembly members:
entity_1, polymer, 213 residues, 24357 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet-1

Data sets:
Data typeCount
13C chemical shifts528
15N chemical shifts198
1H chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Larp7 extended xRRM2 domain1

Entities:

Entity 1, Larp7 extended xRRM2 domain 213 residues - 24357 Da.

1   GLYGLUGLUVALILEPROLEUARGVALLEU
2   SERLYSSERGLUTRPMETASPLEULYSLYS
3   GLUTYRLEUALALEUGLNLYSALASERMET
4   ALASERLEULYSLYSTHRILESERGLNILE
5   LYSSERGLUSERGLUMETGLUTHRASPSER
6   GLYVALPROGLNASNTHRGLYMETLYSASN
7   GLULYSTHRALAASNARGGLUGLUCYSARG
8   THRGLNGLULYSVALASNALATHRGLYPRO
9   GLNPHEVALSERGLYVALILEVALLYSILE
10   ILESERTHRGLUPROLEUPROGLYARGLYS
11   GLNVALARGASPTHRLEUALAALAILESER
12   GLUVALLEUTYRVALASPLEULEUGLUGLY
13   ASPTHRGLUCYSHISALAARGPHELYSTHR
14   PROGLUASPALAGLNALAVALILEASNALA
15   TYRTHRGLUILEASNLYSLYSHISCYSTRP
16   LYSLEUGLUILELEUSERGLYASPHISGLU
17   GLNARGTYRTRPGLNLYSILELEUVALASP
18   ARGGLNALALYSLEUASNGLNPROARGGLU
19   LYSLYSARGGLYTHRGLULYSLEUILETHR
20   LYSALAGLULYSILEARGLEUALALYSTHR
21   GLNGLNALASERLYSHISILEARGPHESER
22   GLUTYRASP

Samples:

sample_1: Larp7 extended xRRM2 domain, [U-13C; U-15N], 0.2 mM; sodium phosphate 20 mM; potassium chloride 50 mM; TCEP 1 mM; D2O 5%

sample_conditions_1: ionic strength: 0.07 M; pH: 6.13; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CCONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN vv4.0.7 - collection

TOPSPIN vv3.5pl7 - collection

NMRPipe vv2016.273.11.31 - processing

NMRFAM-SPARKY vv1.470 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz
  • Bruker AVANCE III HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks