BMRB Entry 51249

Name: 1H and 15N Chemical Shift Assignments for T4 RNA
Published: 2022-01-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51249

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H and 15N Chemical Shift Assignments for T4 RNA

Deposition date:

2021-12-21

Original release date:

2022-01-14

Authors:

Han, Ge; Xue, Yi

Citation:

Citation: Han, Ge; Xue, Yi. "Rational design of hairpin RNA excited states reveals multi-step transitions" Nat. Commun. 13, 1523-1523 (2022).
PubMed: 35314698

Assembly members:

Assembly members:
entity_1, polymer, 29 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: in vitro transcription Host organism: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGAAGUGCGCGCUUCGGCG CGCCUUUCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	13
1H chemical shifts	13

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T4 RNA	1

Entities:

Entity 1, T4 RNA 29 residues - Formula weight is not available

1

G

A

G

U

G

C

G

2

C

G

C

U

C

G

C

G

3

C

G

C

U

C

Samples:

sample_1: T4 RNA 2.90 mM; sodium phosphate 10 mM; EDTA 0.01 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 6.4; pressure: 1 atm; temperature: 283.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III HD 600 MHz MHz