BMRB Entry 51245

Title:
1H and 15N Chemical Shift Assignments for T1-add2bp RNA
Deposition date:
2021-12-21
Original release date:
2022-01-14
Authors:
Han, Ge; Xue, Yi
Citation:

Citation: Han, Ge; Xue, Yi. "Rational design of hairpin RNA excited states reveals multi-step transitions"  Nat. Commun. 13, 1523-1523 (2022).
PubMed: 35314698

Assembly members:

Assembly members:
entity_1, polymer, 38 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: in vitro transcription   Host organism: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCGCGAAAGGGGAAGGGCA ACUUUUCUAAAACGCGCC

Data sets:
Data typeCount
15N chemical shifts13
1H chemical shifts13

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1T1-add2bp RNA1

Entities:

Entity 1, T1-add2bp RNA 38 residues - Formula weight is not available

1   GGCGCGAAAG
2   GGGAAGGGCA
3   ACUUUUCUAA
4   AACGCGCC

Samples:

sample_1: T1-add2bp RNA 3.00 mM; sodium phosphate 10 mM; EDTA 0.01 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 6.4; pressure: 1 atm; temperature: 283.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HMQCsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III HD 600 MHz MHz