BMRB Entry 51245

Name: 1H and 15N Chemical Shift Assignments for T1-add2bp RNA
Published: 2022-01-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51245

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical Shift Assignments for T1-add2bp RNA

Deposition date:

2021-12-21

Original release date:

2022-01-14

Authors:

Han, Ge; Xue, Yi

Citation:

Citation: Han, Ge; Xue, Yi. "Rational design of hairpin RNA excited states reveals multi-step transitions" Nat. Commun. 13, 1523-1523 (2022).
PubMed: 35314698

Assembly members:

Assembly members:
entity_1, polymer, 38 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: in vitro transcription Host organism: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCGCGAAAGGGGAAGGGCA ACUUUUCUAAAACGCGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	13
1H chemical shifts	13

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T1-add2bp RNA	1

Entities:

Entity 1, T1-add2bp RNA 38 residues - Formula weight is not available

1

G

C

G

C

G

A

G

2

G

A

G

C

A

3

A

C

U

C

U

A

4

A

C

G

C

G

C

Samples:

sample_1: T1-add2bp RNA 3.00 mM; sodium phosphate 10 mM; EDTA 0.01 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 6.4; pressure: 1 atm; temperature: 283.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H ROESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III HD 600 MHz MHz