BMRB Entry 51240

Title:
Backbone resonance assignments of the designed Armadillo repeat protein N(A4)MC(AII) at pH 5.5
Deposition date:
2021-12-20
Original release date:
2022-05-31
Authors:
Michel, Erich
Citation:

Citation: Michel, Erich; Cucuzza, Stefano; Mittl, Peer; Zerbe, Oliver; Pluckthun, Andreas. "Improved Repeat Protein Stability by Combined Consensus and Computational Protein Design"  Biochemistry 62, 318-329 (2023).
PubMed: 35657362

Assembly members:

Assembly members:
entity_1, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEM3BTC

Data sets:
Data typeCount
13C chemical shifts337
15N chemical shifts111
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N(A4)MC(AII)1

Entities:

Entity 1, N(A4)MC(AII) 116 residues - Formula weight is not available

1   GLYPROASPLEUPROLYSLEUVALLYSLEU
2   LEULYSSERSERASNGLUGLUILELEULEU
3   LYSALALEUARGALALEUALAGLUILEALA
4   SERGLYGLYASNGLUGLNILEGLNALAVAL
5   ILEASPALAGLYALALEUPROALALEUVAL
6   GLNLEULEUSERSERPROASNGLUGLNILE
7   LEUGLNGLUALALEUTRPALALEUSERASN
8   ILEALASERGLYGLYASNGLUGLNLYSGLN
9   ALAVALLYSGLUALAGLYALALEUGLULYS
10   LEUGLUGLNLEUGLNSERHISGLUASNGLU
11   LYSILEGLNLYSGLUALAGLNGLUALALEU
12   GLULYSLEUGLNSERHIS

Samples:

sample_1: N(A4)MC(AII), [U-98% 13C; U-98% 15N], 0.8 mM; D2O, [U-100% 2H], 5%; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.092 M; pH: 5.5; pressure: 1 atm; temperature: 310.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks