Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51227
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Citation: Kumari, Khushboo; Sarma, Siddhartha. "Structural and mutational analysis of MazE6-operator DNA complex provide insights into autoregulation of toxin-antitoxin systems" Commun. Biol. 5, 963-963 (2022).
PubMed: 36109664
Assembly members:
entity_1, polymer, 52 residues, Formula weight is not available
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21(a)
Entity Sequences (FASTA):
entity_1: SGSMKTAISLPDETFDRVSR
RASELGMSRSEFFTKAAQRY
LHELDAQLLTGQ
Data type | Count |
13C chemical shifts | 180 |
15N chemical shifts | 53 |
1H chemical shifts | 275 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Chain A | 1 |
2 | Chain B | 1 |
Entity 1, Chain A 52 residues - Formula weight is not available
Three residues (SGS) at the start of this sequence come from the affinity tag, and they have been numbered as -2, -1, and 0 respectively.
1 | SER | GLY | SER | MET | LYS | THR | ALA | ILE | SER | LEU | ||||
2 | PRO | ASP | GLU | THR | PHE | ASP | ARG | VAL | SER | ARG | ||||
3 | ARG | ALA | SER | GLU | LEU | GLY | MET | SER | ARG | SER | ||||
4 | GLU | PHE | PHE | THR | LYS | ALA | ALA | GLN | ARG | TYR | ||||
5 | LEU | HIS | GLU | LEU | ASP | ALA | GLN | LEU | LEU | THR | ||||
6 | GLY | GLN |
sample_1: nMazE6 400 uM; potassium phosphate 20 mM; sodium chloride 20 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.2 mM; D2O, [U-99% 2H], 10%
sample_2: nMazE6, [U-99% 15N], 400 uM; potassium phosphate 20 mM; sodium chloride 20 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.2 mM; D2O, [U-99% 2H], 10%
sample_3: nMazE6, [U-99% 13C; U-99% 15N], 400 uM; potassium phosphate 20 mM; sodium chloride 20 mM; EDTA 1 mM; sodium azide 0.01%; DSS 0.2 mM; D2O, [U-99% 2H], 10%
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
3D CC(CO)NH | sample_3 | isotropic | sample_conditions_1 |
3D HNCO | sample_3 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_3 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_3 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
13C, 15N simultaneous edited NOESY | sample_3 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
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