BMRB Entry 51224

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51224

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Title:
1H15N HSQC chemical shifts and Relaxation Parameters for Y99C MIF Mixed Trimers
Deposition date:
2021-12-07
Original release date:
2022-03-09
Authors:
Skeens, Erin; Lisi, George
Citation:

Citation: Skeens, Erin; Pantouris, Georgios; Shah, Dilip; Manjula, Ramu; Ombrello, Michael; Maluf, N.; Bhandari, Vineet; Lisi, George; Lolis, Elias. "A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies"  Front. Mol. Biosci. 9, 783669-783669 (2022).
PubMed: 35252348

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available
entity_2, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PMFIVNTNVPRASVPDGFLS ELTQQLAQATGKPPQYIAVH VVPDQLMAFGGSSEPCALCS LHSIGKIGGAQNRSYSKLLC GLLAERLRISPDRVYINYCD MNAANVGWNNSTFA
entity_2: PMFIVNTNVPRASVPDGFLS ELTQQLAQATGKPPQYIAVH VVPDQLMAFGGSSEPCALCS LHSIGKIGGAQNRSYSKLLC GLLAERLRISPDRVYINYYD MNAANVGWNNSTFA

Data sets:
Data typeCount
15N chemical shifts95
1H chemical shifts95
T1 relaxation values81
T2 relaxation values84
heteronuclear NOE values78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Y99C MIF monomer1
2WT MIF monomer2

Entities:

Entity 1, Y99C MIF monomer 114 residues - Formula weight is not available

1   PROMETPHEILEVALASNTHRASNVALPRO
2   ARGALASERVALPROASPGLYPHELEUSER
3   GLULEUTHRGLNGLNLEUALAGLNALATHR
4   GLYLYSPROPROGLNTYRILEALAVALHIS
5   VALVALPROASPGLNLEUMETALAPHEGLY
6   GLYSERSERGLUPROCYSALALEUCYSSER
7   LEUHISSERILEGLYLYSILEGLYGLYALA
8   GLNASNARGSERTYRSERLYSLEULEUCYS
9   GLYLEULEUALAGLUARGLEUARGILESER
10   PROASPARGVALTYRILEASNTYRCYSASP
11   METASNALAALAASNVALGLYTRPASNASN
12   SERTHRPHEALA

Entity 2, WT MIF monomer 114 residues - Formula weight is not available

1   PROMETPHEILEVALASNTHRASNVALPRO
2   ARGALASERVALPROASPGLYPHELEUSER
3   GLULEUTHRGLNGLNLEUALAGLNALATHR
4   GLYLYSPROPROGLNTYRILEALAVALHIS
5   VALVALPROASPGLNLEUMETALAPHEGLY
6   GLYSERSERGLUPROCYSALALEUCYSSER
7   LEUHISSERILEGLYLYSILEGLYGLYALA
8   GLNASNARGSERTYRSERLYSLEULEUCYS
9   GLYLEULEUALAGLUARGLEUARGILESER
10   PROASPARGVALTYRILEASNTYRTYRASP
11   METASNALAALAASNVALGLYTRPASNASN
12   SERTHRPHEALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks