BMRB Entry 51208

Name: Musashi-2 C terminal
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51208

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Musashi-2 C terminal

Deposition date:

2021-12-02

Original release date:

2022-05-11

Authors:

Huang, Jie-rong

Citation:

Citation: Chiu, Shih-Hui; Ho, Wen-Lin; Sun, Yung-Chen; Kuo, Jean-Cheng; Huang, Jie-Rong. "Phase separation driven by interchangeable properties in the intrinsically disordered regions of protein paralogs" Commun. Biol. 5, 400-400 (2022).
PubMed: 35487971

Assembly members:

Assembly members:
entity_1, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPSYGYQFPGFPAAAYGPVA AAAVAAARGSGSNPARPGGF PGANSPGPVADLYGPASQDS GVGNYISAASPQPGSGFGHG IAGPLIATAFTNGYHHHHHH H

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	262
15N chemical shifts	81
1H chemical shifts	81

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Musashi-2 C terminal, msi2	1

Entities:

Entity 1, Musashi-2 C terminal, msi2 101 residues - Formula weight is not available

1

MET

PRO

SER

TYR

GLY

TYR

GLN

PHE

PRO

GLY

2

PHE

PRO

ALA

TYR

GLY

PRO

VAL

ALA

3

ALA

VAL

ALA

ARG

GLY

SER

4

GLY

SER

ASN

PRO

ALA

ARG

PRO

GLY

PHE

5

PRO

GLY

ALA

ASN

SER

PRO

GLY

PRO

VAL

ALA

6

ASP

LEU

TYR

GLY

PRO

ALA

SER

GLN

ASP

SER

7

GLY

VAL

GLY

ASN

TYR

ILE

SER

ALA

SER

8

PRO

GLN

PRO

GLY

SER

GLY

PHE

GLY

HIS

GLY

9

ILE

ALA

GLY

PRO

LEU

ILE

ALA

THR

ALA

PHE

10

THR

ASN

GLY

TYR

HIS

11

HIS

Samples:

sample_1: Musashi-2 C terminal domain, [U-98% 13C; U-98% 15N], 100 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks