BMRB Entry 51205

Name: Musashi-1 C terminal deltaSeq1
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51205

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Musashi-1 C terminal deltaSeq1

Deposition date:

2021-12-02

Original release date:

2022-05-11

Authors:

Huang, Jie-rong

Citation:

Citation: Chiu, Shih-Hui; Ho, Wen-Lin; Sun, Yung-Chen; Kuo, Jean-Cheng; Huang, Jie-Rong. "Phase separation driven by interchangeable properties in the intrinsically disordered regions of protein paralogs" Commun. Biol. 5, 400-400 (2022).
PubMed: 35487971

Assembly members:

Assembly members:
entity_1, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPGYTYQFPEFRVERTPLPS APVLPELTAIPLTAYGPMAA AAAAAAVVRGTGSHPWTMAP PPGSTPSRTGGFLGTTSPGP MAELYGAANQDSGVSSYISA ASPAPSTGFGHSLGGPLIAT AFTNGYH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	324
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Musashi-1 C terminal deltaSeq1, msi1_ds2	1

Entities:

Entity 1, Musashi-1 C terminal deltaSeq1, msi1_ds2 127 residues - Formula weight is not available

1

MET

PRO

GLY

TYR

THR

TYR

GLN

PHE

PRO

GLU

2

PHE

ARG

VAL

GLU

ARG

THR

PRO

LEU

PRO

SER

3

ALA

PRO

VAL

LEU

PRO

GLU

LEU

THR

ALA

ILE

4

PRO

LEU

THR

ALA

TYR

GLY

PRO

MET

ALA

5

ALA

VAL

ARG

GLY

6

THR

GLY

SER

HIS

PRO

TRP

THR

MET

ALA

PRO

7

PRO

GLY

SER

THR

PRO

SER

ARG

THR

GLY

8

GLY

PHE

LEU

GLY

THR

SER

PRO

GLY

PRO

9

MET

ALA

GLU

LEU

TYR

GLY

ALA

ASN

GLN

10

ASP

SER

GLY

VAL

SER

TYR

ILE

SER

ALA

11

ALA

SER

PRO

ALA

PRO

SER

THR

GLY

PHE

GLY

12

HIS

SER

LEU

GLY

PRO

LEU

ILE

ALA

THR

13

ALA

PHE

THR

ASN

GLY

TYR

HIS

Samples:

sample_1: Musashi-1 C terminal domain delta-seq1, [U-98% 13C; U-98% 15N], 100 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks