BMRB Entry 51204

Name: Musashi-1 C terminal deltaSeq1
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51204

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Musashi-1 C terminal deltaSeq1

Deposition date:

2021-12-02

Original release date:

2022-05-11

Authors:

Huang, Jie-rong

Citation:

Citation: Chiu, Shih-Hui; Ho, Wen-Lin; Sun, Yung-Chen; Kuo, Jean-Cheng; Huang, Jie-Rong. "Phase separation driven by interchangeable properties in the intrinsically disordered regions of protein paralogs" Commun. Biol. 5, 400-400 (2022).
PubMed: 35487971

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPGYTYQFPEFPLTAYGPMA AAAAAAAVVRGTGSHPWTMA PPPGSTPSRTGGFLGTTSPG PMAELYGAANQDSGVSSYIS AASPAPSTGFGHSLGGPLIA TAFTNGYHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	289
15N chemical shifts	91
1H chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Musashi-1 C terminal deltaSeq1, msi1_ds1	1

Entities:

Entity 1, Musashi-1 C terminal deltaSeq1, msi1_ds1 114 residues - Formula weight is not available

1

MET

PRO

GLY

TYR

THR

TYR

GLN

PHE

PRO

GLU

2

PHE

PRO

LEU

THR

ALA

TYR

GLY

PRO

MET

ALA

3

ALA

VAL

ARG

4

GLY

THR

GLY

SER

HIS

PRO

TRP

THR

MET

ALA

5

PRO

GLY

SER

THR

PRO

SER

ARG

THR

6

GLY

PHE

LEU

GLY

THR

SER

PRO

GLY

7

PRO

MET

ALA

GLU

LEU

TYR

GLY

ALA

ASN

8

GLN

ASP

SER

GLY

VAL

SER

TYR

ILE

SER

9

ALA

SER

PRO

ALA

PRO

SER

THR

GLY

PHE

10

GLY

HIS

SER

LEU

GLY

PRO

LEU

ILE

ALA

11

THR

ALA

PHE

THR

ASN

GLY

TYR

HIS

12

HIS

Samples:

sample_1: Musashi-1 C terminal domain delta-seq1, [U-98% 13C; U-98% 15N], 100 uM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks