BMRB Entry 5120
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5120
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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RDF gzip file.
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Citation: Chamberlin, S.; Brennan, L.; Puddicombe, S.; Davies, D.; Turner, D.. "Solution Structure of the mEGF/TGFalpha44-50 Chimeric Growth Factor" Eur. J. Biochem. 268, 6247-6255 (2001).
PubMed: 11733021
Assembly members:
mEGF/TGFalpha44-50 chimera, polymer, 49 residues, 5000 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
mEGF/TGFalpha44-50 chimera: NSYPGCPSSYDGYCLNGGVC
MHIESLDSYTCNCVIGYSGD
RCEHADLLA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 268 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mEGF/TGFalpha44-50 chimera | 1 |
Entities:
Entity 1, mEGF/TGFalpha44-50 chimera 49 residues - 5000 Da.
| 1 | ASN | SER | TYR | PRO | GLY | CYS | PRO | SER | SER | TYR | ||||
| 2 | ASP | GLY | TYR | CYS | LEU | ASN | GLY | GLY | VAL | CYS | ||||
| 3 | MET | HIS | ILE | GLU | SER | LEU | ASP | SER | TYR | THR | ||||
| 4 | CYS | ASN | CYS | VAL | ILE | GLY | TYR | SER | GLY | ASP | ||||
| 5 | ARG | CYS | GLU | HIS | ALA | ASP | LEU | LEU | ALA |
Samples:
sample_1: mEGF/TGFalpha44-50 chimera 3 mM
sample_cond_1: pH: 3.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-1H-NOESY | not available | not available | not available |
| 2D-1H-TOCSY | not available | not available | not available |
| 2D-1H-COSY | not available | not available | not available |
Software:
XEASY -
NMR spectrometers:
- VARIAN VXR 500 MHz
Related Database Links:
| PDB |