BMRB Entry 51197

Title:
1H, 15N Backbone Assignments of Rec3 from GeoCas9
Deposition date:
2021-11-29
Original release date:
2024-07-22
Authors:
Belato, Helen
Citation:

Citation: Belato, Helen; Knight, Alexa; D'Ordine, Alexandra; Fan, Zhiqiang; Luo, Jinping; Jogl, Gerwald; Lisi, George. "Atomistic Tuning of the GeoCas9 Recognition Lobe Modulates Allosteric Motions and Guide RNA Interactions"  bioRxiv ., .-. (2024).
PubMed: 38746279

Assembly members:

Assembly members:
entity_1, polymer, 212 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rec31

Entities:

Entity 1, Rec3 212 residues - Formula weight is not available

1   LYSGLULYSARGALAPROLYSALATHRTYR
2   THRPHEGLNSERPHEILEALATRPGLUHIS
3   ILEASNLYSLEUARGLEUILESERPROSER
4   GLYALAARGGLYLEUTHRASPGLUGLUARG
5   ARGLEULEUTYRGLUGLNALAPHEGLNLYS
6   ASNLYSILETHRTYRHISASPILEARGTHR
7   LEULEUHISLEUPROASPASPTHRTYRPHE
8   LYSGLYILEVALTYRASPARGGLYGLUSER
9   ARGLYSGLNASNGLUASNILEARGPHELEU
10   GLULEUASPALATYRHISGLNILEARGLYS
11   ALAVALASPLYSVALTYRGLYLYSGLYLYS
12   SERSERSERPHELEUPROILEASPPHEASP
13   THRPHEGLYTYRALALEUTHRLEUPHELYS
14   ASPASPALAASPILEHISSERTYRLEUARG
15   ASNGLUTYRGLUGLNASNGLYLYSARGMET
16   PROASNLEUALAASNLYSVALTYRASPASN
17   GLULEUILEGLUGLULEULEUASNLEUSER
18   PHETHRLYSPHEGLYHISLEUSERLEULYS
19   ALALEUARGSERILELEUPROTYRMETGLU
20   GLNGLYGLUVALTYRSERSERALACYSGLU
21   ARGALAGLYTYRTHRPHETHRGLYPROLYS
22   LYSLYS

Samples:

sample_1: Rec3, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 20 mM; potassium chloride 80 mM; DTT 1 mM; EDTA 1 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks