BMRB Entry 51182

Title:
Human Amylin HNa and HNz PEGylated (Bis-PEGylated)
Deposition date:
2021-11-16
Original release date:
2021-12-01
Authors:
de Araujo, Talita; Lima, Luis Mauricio; Almeida, Marcius
Citation:

Citation: Lima, Luis Mauricio; de Araujo, Talita; Almeida, Marcius. "Unambiguous characterization of PEGylation site on human amylin by 2D NMR"  Peptide Science ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available
entity_PEG, non-polymer, 106.120 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:
Data typeCount
13C chemical shifts99
1H chemical shifts229

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Amylin1
2polyethylene glycol2

Entities:

Entity 1, Human Amylin 37 residues - Formula weight is not available

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Entity 2, polyethylene glycol - C4 H10 O3 - 106.120 Da.

1   PEG

Samples:

sample_1: Human amylin 0.5 mM; sodium acetate 10 mM

sample_conditions_1: pH: 4.5; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN vv4.0.6 - collection, data analysis, processing

CARA vv1.9.1.5 - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Ascend 500 MHz