BMRB Entry 51178
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51178
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tsunaka, Yasuo; Ohtomo, Hideaki; Nishimura, Yoshifumi. "FACT modulates the conformations of histone H2A and H2B N-terminal tails within nucleosomes" Commun. Biol. 5, 814-814 (2022).
PubMed: 35963897
Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
entity_2, polymer, 129 residues, Formula weight is not available
entity_3, polymer, 135 residues, Formula weight is not available
entity_4, polymer, 102 residues, Formula weight is not available
entity_5, polymer, 112 residues, Formula weight is not available
entity_6, polymer, 112 residues, Formula weight is not available
entity_7, polymer, 41 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
entity_1: GPGMSGRGKQGGKARAKAKT
RSSRAGLQFPVGRVHRLLRK
GNYSERVGAGAPVYLAAVLE
YLTAEILELAGNAARDNKKT
RIIPRHLQLAIRNDEELNKL
LGRVTIAQGGVLPNIQAVLL
PKKTESHHKAKGK
entity_2: GPGMPEPAKSAPAPKKGSKK
AVTKAQKKDGKKRKRSRKES
YSIYVYKVLKQVHPDTGISS
KAMGIMNSFVNDIFERIAGE
ASRLAHYNKRSTITSREIQT
AVRLLLPGELAKHAVSEGTK
AVTKYTSAK
entity_3: ARTKQTARKSTGGKAPRKQL
ATKAARKSAPATGGVKKPHR
YRPGTVALREIRRYQKSTEL
LIRKLPFQRLVREIAQDFKT
DLRFQSSAVMALQEACEAYL
VGLFEDTNLCAIHAKRVTIM
PKDIQLARRIRGERA
entity_4: SGRGKGGKGLGKGGAKRHRK
VLRDNIQGITKPAIRRLARR
GGVKRISGLIYEETRGVLKV
FLENVIRDAVTYTEHAKRKT
VTAMDVVYALKRQGRTLYGF
GG
entity_5: ATCTAGACAGCTCTAGCACC
GCTTAAACGCACGTACGCGC
TGTCCCCCGCGTTTTAACCG
CCAAGGGGATTACTCCCTAG
TCTCCAGGCACGTGTCAGAT
ATATACATCGAT
entity_6: ATCGATGTATATATCTGACA
CGTGCCTGGAGACTAGGGAG
TAATCCCCTTGGCGGTTAAA
ACGCGGGGGACAGCGCGTAC
GTGCGTTTAAGCGGTGCTAG
AGCTGTCTAGAT
entity_7: HMEPEGEGSDAEEGDSESEI
EDETFNPSEDDYEEEEEDSD
E
- assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2
- assigned_chemical_shifts_3
- assigned_chemical_shifts_4
- assigned_chemical_shifts_5
- assigned_chemical_shifts_6
- assigned_chemical_shifts_7
- assigned_chemical_shifts_8
- assigned_chemical_shifts_9
- assigned_chemical_shifts_10
- assigned_chemical_shifts_11
- assigned_chemical_shifts_12
- assigned_chemical_shifts_13
- assigned_chemical_shifts_14
- assigned_chemical_shifts_15
- assigned_chemical_shifts_16
- assigned_chemical_shifts_17
- assigned_chemical_shifts_18
- assigned_chemical_shifts_19
- assigned_chemical_shifts_20
- assigned_chemical_shifts_21
- assigned_chemical_shifts_22
- assigned_chemical_shifts_23
- assigned_chemical_shifts_24
- assigned_chemical_shifts_25
| Data type | Count |
| 13C chemical shifts | 223 |
| 15N chemical shifts | 308 |
| 1H chemical shifts | 308 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hsitone H2A_1 | 1 |
| 2 | Hsitone H2A_2 | 1 |
| 3 | Histone H2B_1 | 2 |
| 4 | Histone H2B_2 | 2 |
| 5 | Histone H3.1_1 | 3 |
| 6 | Histone H3.1_2 | 3 |
| 7 | Histone H4_1 | 4 |
| 8 | Histone H4_2 | 4 |
| 9 | 112-bp DNA_1 | 5 |
| 10 | 112-bp DNA_2 | 6 |
| 11 | FACT_pAID | 7 |
Entities:
Entity 1, Hsitone H2A_1 133 residues - Formula weight is not available
| 1 | GLY | PRO | GLY | MET | SER | GLY | ARG | GLY | LYS | GLN | ||||
| 2 | GLY | GLY | LYS | ALA | ARG | ALA | LYS | ALA | LYS | THR | ||||
| 3 | ARG | SER | SER | ARG | ALA | GLY | LEU | GLN | PHE | PRO | ||||
| 4 | VAL | GLY | ARG | VAL | HIS | ARG | LEU | LEU | ARG | LYS | ||||
| 5 | GLY | ASN | TYR | SER | GLU | ARG | VAL | GLY | ALA | GLY | ||||
| 6 | ALA | PRO | VAL | TYR | LEU | ALA | ALA | VAL | LEU | GLU | ||||
| 7 | TYR | LEU | THR | ALA | GLU | ILE | LEU | GLU | LEU | ALA | ||||
| 8 | GLY | ASN | ALA | ALA | ARG | ASP | ASN | LYS | LYS | THR | ||||
| 9 | ARG | ILE | ILE | PRO | ARG | HIS | LEU | GLN | LEU | ALA | ||||
| 10 | ILE | ARG | ASN | ASP | GLU | GLU | LEU | ASN | LYS | LEU | ||||
| 11 | LEU | GLY | ARG | VAL | THR | ILE | ALA | GLN | GLY | GLY | ||||
| 12 | VAL | LEU | PRO | ASN | ILE | GLN | ALA | VAL | LEU | LEU | ||||
| 13 | PRO | LYS | LYS | THR | GLU | SER | HIS | HIS | LYS | ALA | ||||
| 14 | LYS | GLY | LYS |
Entity 2, Histone H2B_1 129 residues - Formula weight is not available
| 1 | GLY | PRO | GLY | MET | PRO | GLU | PRO | ALA | LYS | SER | ||||
| 2 | ALA | PRO | ALA | PRO | LYS | LYS | GLY | SER | LYS | LYS | ||||
| 3 | ALA | VAL | THR | LYS | ALA | GLN | LYS | LYS | ASP | GLY | ||||
| 4 | LYS | LYS | ARG | LYS | ARG | SER | ARG | LYS | GLU | SER | ||||
| 5 | TYR | SER | ILE | TYR | VAL | TYR | LYS | VAL | LEU | LYS | ||||
| 6 | GLN | VAL | HIS | PRO | ASP | THR | GLY | ILE | SER | SER | ||||
| 7 | LYS | ALA | MET | GLY | ILE | MET | ASN | SER | PHE | VAL | ||||
| 8 | ASN | ASP | ILE | PHE | GLU | ARG | ILE | ALA | GLY | GLU | ||||
| 9 | ALA | SER | ARG | LEU | ALA | HIS | TYR | ASN | LYS | ARG | ||||
| 10 | SER | THR | ILE | THR | SER | ARG | GLU | ILE | GLN | THR | ||||
| 11 | ALA | VAL | ARG | LEU | LEU | LEU | PRO | GLY | GLU | LEU | ||||
| 12 | ALA | LYS | HIS | ALA | VAL | SER | GLU | GLY | THR | LYS | ||||
| 13 | ALA | VAL | THR | LYS | TYR | THR | SER | ALA | LYS |
Entity 3, Histone H3.1_1 135 residues - Formula weight is not available
| 1 | ALA | ARG | THR | LYS | GLN | THR | ALA | ARG | LYS | SER | ||||
| 2 | THR | GLY | GLY | LYS | ALA | PRO | ARG | LYS | GLN | LEU | ||||
| 3 | ALA | THR | LYS | ALA | ALA | ARG | LYS | SER | ALA | PRO | ||||
| 4 | ALA | THR | GLY | GLY | VAL | LYS | LYS | PRO | HIS | ARG | ||||
| 5 | TYR | ARG | PRO | GLY | THR | VAL | ALA | LEU | ARG | GLU | ||||
| 6 | ILE | ARG | ARG | TYR | GLN | LYS | SER | THR | GLU | LEU | ||||
| 7 | LEU | ILE | ARG | LYS | LEU | PRO | PHE | GLN | ARG | LEU | ||||
| 8 | VAL | ARG | GLU | ILE | ALA | GLN | ASP | PHE | LYS | THR | ||||
| 9 | ASP | LEU | ARG | PHE | GLN | SER | SER | ALA | VAL | MET | ||||
| 10 | ALA | LEU | GLN | GLU | ALA | CYS | GLU | ALA | TYR | LEU | ||||
| 11 | VAL | GLY | LEU | PHE | GLU | ASP | THR | ASN | LEU | CYS | ||||
| 12 | ALA | ILE | HIS | ALA | LYS | ARG | VAL | THR | ILE | MET | ||||
| 13 | PRO | LYS | ASP | ILE | GLN | LEU | ALA | ARG | ARG | ILE | ||||
| 14 | ARG | GLY | GLU | ARG | ALA |
Entity 4, Histone H4_1 102 residues - Formula weight is not available
| 1 | SER | GLY | ARG | GLY | LYS | GLY | GLY | LYS | GLY | LEU | ||||
| 2 | GLY | LYS | GLY | GLY | ALA | LYS | ARG | HIS | ARG | LYS | ||||
| 3 | VAL | LEU | ARG | ASP | ASN | ILE | GLN | GLY | ILE | THR | ||||
| 4 | LYS | PRO | ALA | ILE | ARG | ARG | LEU | ALA | ARG | ARG | ||||
| 5 | GLY | GLY | VAL | LYS | ARG | ILE | SER | GLY | LEU | ILE | ||||
| 6 | TYR | GLU | GLU | THR | ARG | GLY | VAL | LEU | LYS | VAL | ||||
| 7 | PHE | LEU | GLU | ASN | VAL | ILE | ARG | ASP | ALA | VAL | ||||
| 8 | THR | TYR | THR | GLU | HIS | ALA | LYS | ARG | LYS | THR | ||||
| 9 | VAL | THR | ALA | MET | ASP | VAL | VAL | TYR | ALA | LEU | ||||
| 10 | LYS | ARG | GLN | GLY | ARG | THR | LEU | TYR | GLY | PHE | ||||
| 11 | GLY | GLY |
Entity 5, 112-bp DNA_1 112 residues - Formula weight is not available
| 1 | DA | DT | DC | DT | DA | DG | DA | DC | DA | DG | ||||
| 2 | DC | DT | DC | DT | DA | DG | DC | DA | DC | DC | ||||
| 3 | DG | DC | DT | DT | DA | DA | DA | DC | DG | DC | ||||
| 4 | DA | DC | DG | DT | DA | DC | DG | DC | DG | DC | ||||
| 5 | DT | DG | DT | DC | DC | DC | DC | DC | DG | DC | ||||
| 6 | DG | DT | DT | DT | DT | DA | DA | DC | DC | DG | ||||
| 7 | DC | DC | DA | DA | DG | DG | DG | DG | DA | DT | ||||
| 8 | DT | DA | DC | DT | DC | DC | DC | DT | DA | DG | ||||
| 9 | DT | DC | DT | DC | DC | DA | DG | DG | DC | DA | ||||
| 10 | DC | DG | DT | DG | DT | DC | DA | DG | DA | DT | ||||
| 11 | DA | DT | DA | DT | DA | DC | DA | DT | DC | DG | ||||
| 12 | DA | DT |
Entity 6, 112-bp DNA_2 112 residues - Formula weight is not available
| 1 | DA | DT | DC | DG | DA | DT | DG | DT | DA | DT | ||||
| 2 | DA | DT | DA | DT | DC | DT | DG | DA | DC | DA | ||||
| 3 | DC | DG | DT | DG | DC | DC | DT | DG | DG | DA | ||||
| 4 | DG | DA | DC | DT | DA | DG | DG | DG | DA | DG | ||||
| 5 | DT | DA | DA | DT | DC | DC | DC | DC | DT | DT | ||||
| 6 | DG | DG | DC | DG | DG | DT | DT | DA | DA | DA | ||||
| 7 | DA | DC | DG | DC | DG | DG | DG | DG | DG | DA | ||||
| 8 | DC | DA | DG | DC | DG | DC | DG | DT | DA | DC | ||||
| 9 | DG | DT | DG | DC | DG | DT | DT | DT | DA | DA | ||||
| 10 | DG | DC | DG | DG | DT | DG | DC | DT | DA | DG | ||||
| 11 | DA | DG | DC | DT | DG | DT | DC | DT | DA | DG | ||||
| 12 | DA | DT |
Entity 7, FACT_pAID 41 residues - Formula weight is not available
| 1 | HIS | MET | GLU | PRO | GLU | GLY | GLU | GLY | SER | ASP | ||||
| 2 | ALA | GLU | GLU | GLY | ASP | SER | GLU | SER | GLU | ILE | ||||
| 3 | GLU | ASP | GLU | THR | PHE | ASN | PRO | SER | GLU | ASP | ||||
| 4 | ASP | TYR | GLU | GLU | GLU | GLU | GLU | ASP | SER | ASP | ||||
| 5 | GLU |
Samples:
sample_1: Histone H2A, [U-100% 13C; U-100% 15N; U-80% 2H], 50 uM; Histone H2B, [U-100% 13C; U-100% 15N; U-80% 2H], 50 uM; HEPES 20 mM; sodium chloride 0-300 mM; Histone H3.1 50 uM; Histone H4 50 uM; FACT_pAID 25 uM; 112-bp 601 DNA_1 25 uM; 112-bp 601 DNA_2 25 uM
sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
sample_conditions_2: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
sample_conditions_3: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
sample_conditions_4: ionic strength: 200 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
sample_conditions_5: ionic strength: 300 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_3 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_4 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_5 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
Olivia - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks