BMRB Entry 51178

Title:
Histone H2A and H2B tails within 112-bp DNA/pAID nucleosome
Deposition date:
2021-11-15
Original release date:
2022-10-12
Authors:
Tsunaka, Yasuo; Ohtomo, Hideaki; Nishimura, Yoshifumi
Citation:

Citation: Tsunaka, Yasuo; Ohtomo, Hideaki; Nishimura, Yoshifumi. "FACT modulates the conformations of histone H2A and H2B N-terminal tails within nucleosomes"  Commun. Biol. 5, 814-814 (2022).
PubMed: 35963897

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
entity_2, polymer, 129 residues, Formula weight is not available
entity_3, polymer, 135 residues, Formula weight is not available
entity_4, polymer, 102 residues, Formula weight is not available
entity_5, polymer, 112 residues, Formula weight is not available
entity_6, polymer, 112 residues, Formula weight is not available
entity_7, polymer, 41 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data typeCount
13C chemical shifts223
15N chemical shifts308
1H chemical shifts308

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsitone H2A_11
2Hsitone H2A_21
3Histone H2B_12
4Histone H2B_22
5Histone H3.1_13
6Histone H3.1_23
7Histone H4_14
8Histone H4_24
9112-bp DNA_15
10112-bp DNA_26
11FACT_pAID7

Entities:

Entity 1, Hsitone H2A_1 133 residues - Formula weight is not available

1   GLYPROGLYMETSERGLYARGGLYLYSGLN
2   GLYGLYLYSALAARGALALYSALALYSTHR
3   ARGSERSERARGALAGLYLEUGLNPHEPRO
4   VALGLYARGVALHISARGLEULEUARGLYS
5   GLYASNTYRSERGLUARGVALGLYALAGLY
6   ALAPROVALTYRLEUALAALAVALLEUGLU
7   TYRLEUTHRALAGLUILELEUGLULEUALA
8   GLYASNALAALAARGASPASNLYSLYSTHR
9   ARGILEILEPROARGHISLEUGLNLEUALA
10   ILEARGASNASPGLUGLULEUASNLYSLEU
11   LEUGLYARGVALTHRILEALAGLNGLYGLY
12   VALLEUPROASNILEGLNALAVALLEULEU
13   PROLYSLYSTHRGLUSERHISHISLYSALA
14   LYSGLYLYS

Entity 2, Histone H2B_1 129 residues - Formula weight is not available

1   GLYPROGLYMETPROGLUPROALALYSSER
2   ALAPROALAPROLYSLYSGLYSERLYSLYS
3   ALAVALTHRLYSALAGLNLYSLYSASPGLY
4   LYSLYSARGLYSARGSERARGLYSGLUSER
5   TYRSERILETYRVALTYRLYSVALLEULYS
6   GLNVALHISPROASPTHRGLYILESERSER
7   LYSALAMETGLYILEMETASNSERPHEVAL
8   ASNASPILEPHEGLUARGILEALAGLYGLU
9   ALASERARGLEUALAHISTYRASNLYSARG
10   SERTHRILETHRSERARGGLUILEGLNTHR
11   ALAVALARGLEULEULEUPROGLYGLULEU
12   ALALYSHISALAVALSERGLUGLYTHRLYS
13   ALAVALTHRLYSTYRTHRSERALALYS

Entity 3, Histone H3.1_1 135 residues - Formula weight is not available

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLULEU
7   LEUILEARGLYSLEUPROPHEGLNARGLEU
8   VALARGGLUILEALAGLNASPPHELYSTHR
9   ASPLEUARGPHEGLNSERSERALAVALMET
10   ALALEUGLNGLUALACYSGLUALATYRLEU
11   VALGLYLEUPHEGLUASPTHRASNLEUCYS
12   ALAILEHISALALYSARGVALTHRILEMET
13   PROLYSASPILEGLNLEUALAARGARGILE
14   ARGGLYGLUARGALA

Entity 4, Histone H4_1 102 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSGLYLEU
2   GLYLYSGLYGLYALALYSARGHISARGLYS
3   VALLEUARGASPASNILEGLNGLYILETHR
4   LYSPROALAILEARGARGLEUALAARGARG
5   GLYGLYVALLYSARGILESERGLYLEUILE
6   TYRGLUGLUTHRARGGLYVALLEULYSVAL
7   PHELEUGLUASNVALILEARGASPALAVAL
8   THRTYRTHRGLUHISALALYSARGLYSTHR
9   VALTHRALAMETASPVALVALTYRALALEU
10   LYSARGGLNGLYARGTHRLEUTYRGLYPHE
11   GLYGLY

Entity 5, 112-bp DNA_1 112 residues - Formula weight is not available

1   DADTDCDTDADGDADCDADG
2   DCDTDCDTDADGDCDADCDC
3   DGDCDTDTDADADADCDGDC
4   DADCDGDTDADCDGDCDGDC
5   DTDGDTDCDCDCDCDCDGDC
6   DGDTDTDTDTDADADCDCDG
7   DCDCDADADGDGDGDGDADT
8   DTDADCDTDCDCDCDTDADG
9   DTDCDTDCDCDADGDGDCDA
10   DCDGDTDGDTDCDADGDADT
11   DADTDADTDADCDADTDCDG
12   DADT

Entity 6, 112-bp DNA_2 112 residues - Formula weight is not available

1   DADTDCDGDADTDGDTDADT
2   DADTDADTDCDTDGDADCDA
3   DCDGDTDGDCDCDTDGDGDA
4   DGDADCDTDADGDGDGDADG
5   DTDADADTDCDCDCDCDTDT
6   DGDGDCDGDGDTDTDADADA
7   DADCDGDCDGDGDGDGDGDA
8   DCDADGDCDGDCDGDTDADC
9   DGDTDGDCDGDTDTDTDADA
10   DGDCDGDGDTDGDCDTDADG
11   DADGDCDTDGDTDCDTDADG
12   DADT

Entity 7, FACT_pAID 41 residues - Formula weight is not available

1   HISMETGLUPROGLUGLYGLUGLYSERASP
2   ALAGLUGLUGLYASPSERGLUSERGLUILE
3   GLUASPGLUTHRPHEASNPROSERGLUASP
4   ASPTYRGLUGLUGLUGLUGLUASPSERASP
5   GLU

Samples:

sample_1: Histone H2A, [U-100% 13C; U-100% 15N; U-80% 2H], 50 uM; Histone H2B, [U-100% 13C; U-100% 15N; U-80% 2H], 50 uM; HEPES 20 mM; sodium chloride 0-300 mM; Histone H3.1 50 uM; Histone H4 50 uM; FACT_pAID 25 uM; 112-bp 601 DNA_1 25 uM; 112-bp 601 DNA_2 25 uM

sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

sample_conditions_2: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

sample_conditions_3: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

sample_conditions_4: ionic strength: 200 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

sample_conditions_5: ionic strength: 300 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
2D 1H-15N HSQCsample_1isotropicsample_conditions_3
2D 1H-15N HSQCsample_1isotropicsample_conditions_4
2D 1H-15N HSQCsample_1isotropicsample_conditions_5
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

Olivia - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks