BMRB Entry 51177

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51177

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Title:
Histone H2A and H2B tails within 112-bp hexasome
Deposition date:
2021-11-14
Original release date:
2022-10-12
Authors:
Tsunaka, Yasuo; Ohtomo, Hideaki; Nishimura, Yoshifumi
Citation:

Citation: Tsunaka, Yasuo; Ohtomo, Hideaki; Nishimura, Yoshifumi. "FACT modulates the conformations of histone H2A and H2B N-terminal tails within nucleosomes"  Commun. Biol. 5, 814-814 (2022).
PubMed: 35963897

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
entity_2, polymer, 129 residues, Formula weight is not available
entity_3, polymer, 135 residues, Formula weight is not available
entity_4, polymer, 102 residues, Formula weight is not available
entity_5, polymer, 112 residues, Formula weight is not available
entity_6, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGMSGRGKQGGKARAKAKT RSSRAGLQFPVGRVHRLLRK GNYSERVGAGAPVYLAAVLE YLTAEILELAGNAARDNKKT RIIPRHLQLAIRNDEELNKL LGRVTIAQGGVLPNIQAVLL PKKTESHHKAKGK
entity_2: GPGMPEPAKSAPAPKKGSKK AVTKAQKKDGKKRKRSRKES YSIYVYKVLKQVHPDTGISS KAMGIMNSFVNDIFERIAGE ASRLAHYNKRSTITSREIQT AVRLLLPGELAKHAVSEGTK AVTKYTSAK
entity_3: ARTKQTARKSTGGKAPRKQL ATKAARKSAPATGGVKKPHR YRPGTVALREIRRYQKSTEL LIRKLPFQRLVREIAQDFKT DLRFQSSAVMALQEACEAYL VGLFEDTNLCAIHAKRVTIM PKDIQLARRIRGERA
entity_4: SGRGKGGKGLGKGGAKRHRK VLRDNIQGITKPAIRRLARR GGVKRISGLIYEETRGVLKV FLENVIRDAVTYTEHAKRKT VTAMDVVYALKRQGRTLYGF GG
entity_5: ATCTAGACAGCTCTAGCACC GCTTAAACGCACGTACGCGC TGTCCCCCGCGTTTTAACCG CCAAGGGGATTACTCCCTAG TCTCCAGGCACGTGTCAGAT ATATACATCGAT
entity_6: ATCGATGTATATATCTGACA CGTGCCTGGAGACTAGGGAG TAATCCCCTTGGCGGTTAAA ACGCGGGGGACAGCGCGTAC GTGCGTTTAAGCGGTGCTAG AGCTGTCTAGAT

Data typeCount
15N chemical shifts51
1H chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsitone H2A1
2Histone H2B2
3Histone H3.1_13
4Histone H3.1_23
5Histone H4_14
6Histone H4_24
7112-bp DNA_15
8112-bp DNA_26

Entities:

Entity 1, Hsitone H2A 133 residues - Formula weight is not available

1   GLYPROGLYMETSERGLYARGGLYLYSGLN
2   GLYGLYLYSALAARGALALYSALALYSTHR
3   ARGSERSERARGALAGLYLEUGLNPHEPRO
4   VALGLYARGVALHISARGLEULEUARGLYS
5   GLYASNTYRSERGLUARGVALGLYALAGLY
6   ALAPROVALTYRLEUALAALAVALLEUGLU
7   TYRLEUTHRALAGLUILELEUGLULEUALA
8   GLYASNALAALAARGASPASNLYSLYSTHR
9   ARGILEILEPROARGHISLEUGLNLEUALA
10   ILEARGASNASPGLUGLULEUASNLYSLEU
11   LEUGLYARGVALTHRILEALAGLNGLYGLY
12   VALLEUPROASNILEGLNALAVALLEULEU
13   PROLYSLYSTHRGLUSERHISHISLYSALA
14   LYSGLYLYS

Entity 2, Histone H2B 129 residues - Formula weight is not available

1   GLYPROGLYMETPROGLUPROALALYSSER
2   ALAPROALAPROLYSLYSGLYSERLYSLYS
3   ALAVALTHRLYSALAGLNLYSLYSASPGLY
4   LYSLYSARGLYSARGSERARGLYSGLUSER
5   TYRSERILETYRVALTYRLYSVALLEULYS
6   GLNVALHISPROASPTHRGLYILESERSER
7   LYSALAMETGLYILEMETASNSERPHEVAL
8   ASNASPILEPHEGLUARGILEALAGLYGLU
9   ALASERARGLEUALAHISTYRASNLYSARG
10   SERTHRILETHRSERARGGLUILEGLNTHR
11   ALAVALARGLEULEULEUPROGLYGLULEU
12   ALALYSHISALAVALSERGLUGLYTHRLYS
13   ALAVALTHRLYSTYRTHRSERALALYS

Entity 3, Histone H3.1_1 135 residues - Formula weight is not available

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLULEU
7   LEUILEARGLYSLEUPROPHEGLNARGLEU
8   VALARGGLUILEALAGLNASPPHELYSTHR
9   ASPLEUARGPHEGLNSERSERALAVALMET
10   ALALEUGLNGLUALACYSGLUALATYRLEU
11   VALGLYLEUPHEGLUASPTHRASNLEUCYS
12   ALAILEHISALALYSARGVALTHRILEMET
13   PROLYSASPILEGLNLEUALAARGARGILE
14   ARGGLYGLUARGALA

Entity 4, Histone H4_1 102 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSGLYLEU
2   GLYLYSGLYGLYALALYSARGHISARGLYS
3   VALLEUARGASPASNILEGLNGLYILETHR
4   LYSPROALAILEARGARGLEUALAARGARG
5   GLYGLYVALLYSARGILESERGLYLEUILE
6   TYRGLUGLUTHRARGGLYVALLEULYSVAL
7   PHELEUGLUASNVALILEARGASPALAVAL
8   THRTYRTHRGLUHISALALYSARGLYSTHR
9   VALTHRALAMETASPVALVALTYRALALEU
10   LYSARGGLNGLYARGTHRLEUTYRGLYPHE
11   GLYGLY

Entity 5, 112-bp DNA_1 112 residues - Formula weight is not available

1   DADTDCDTDADGDADCDADG
2   DCDTDCDTDADGDCDADCDC
3   DGDCDTDTDADADADCDGDC
4   DADCDGDTDADCDGDCDGDC
5   DTDGDTDCDCDCDCDCDGDC
6   DGDTDTDTDTDADADCDCDG
7   DCDCDADADGDGDGDGDADT
8   DTDADCDTDCDCDCDTDADG
9   DTDCDTDCDCDADGDGDCDA
10   DCDGDTDGDTDCDADGDADT
11   DADTDADTDADCDADTDCDG
12   DADT

Entity 6, 112-bp DNA_2 112 residues - Formula weight is not available

1   DADTDCDGDADTDGDTDADT
2   DADTDADTDCDTDGDADCDA
3   DCDGDTDGDCDCDTDGDGDA
4   DGDADCDTDADGDGDGDADG
5   DTDADADTDCDCDCDCDTDT
6   DGDGDCDGDGDTDTDADADA
7   DADCDGDCDGDGDGDGDGDA
8   DCDADGDCDGDCDGDTDADC
9   DGDTDGDCDGDTDTDTDADA
10   DGDCDGDGDTDGDCDTDADG
11   DADGDCDTDGDTDCDTDADG
12   DADT

Samples:

sample_1: Histone H2A, [U-100% 13C; U-100% 15N; U-80% 2H], 68 uM; Histone H2B, [U-100% 13C; U-100% 15N; U-80% 2H], 68 uM; HEPES 20 mM; Histone H3.1 136 uM; Histone H4 136 uM; 112-bp 601 DNA_1 68 uM; 112-bp 601 DNA_2 68 uM

sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

Olivia - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks