Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51175
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NMR-STAR v3 text file.
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Citation: Pham, ChiL; Titaux-Delgado, GustavoA; Varghese, NikhilR; Polonio, Paula; Wilde, KarynL; Sunde, Margaret; Mompean, Miguel. "NMR characterization of an assembling RHIM (RIP Homotypic Interaction Motif) amyloid reveals a cryptic region for self-recognition" J. Biol. Chem. 299, 104568-104568 (2023).
PubMed: 36870681
Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMAL
Entity Sequences (FASTA):
entity_1: GSSSDSMAQPPQTPETSTFR
NQMPSPTSTGTPSPGPRGNQ
GAERQGMNWSCRTPEPNPVT
GRPLVNIYNCSGVQVGDNNY
LTMQQTTALPTWGLAPSGKG
RGLQHPPPVGSQEGPKDPEA
WSRPQGWYNHSGK
Data type | Count |
13C chemical shifts | 449 |
15N chemical shifts | 126 |
1H chemical shifts | 428 |
T1 relaxation values | 112 |
T1rho relaxation values | 112 |
coupling constants | 84 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | C-terminal domain of RIPK3 | 1 |
Entity 1, C-terminal domain of RIPK3 133 residues - Formula weight is not available
1 | GLY | SER | SER | SER | ASP | SER | MET | ALA | GLN | PRO | ||||
2 | PRO | GLN | THR | PRO | GLU | THR | SER | THR | PHE | ARG | ||||
3 | ASN | GLN | MET | PRO | SER | PRO | THR | SER | THR | GLY | ||||
4 | THR | PRO | SER | PRO | GLY | PRO | ARG | GLY | ASN | GLN | ||||
5 | GLY | ALA | GLU | ARG | GLN | GLY | MET | ASN | TRP | SER | ||||
6 | CYS | ARG | THR | PRO | GLU | PRO | ASN | PRO | VAL | THR | ||||
7 | GLY | ARG | PRO | LEU | VAL | ASN | ILE | TYR | ASN | CYS | ||||
8 | SER | GLY | VAL | GLN | VAL | GLY | ASP | ASN | ASN | TYR | ||||
9 | LEU | THR | MET | GLN | GLN | THR | THR | ALA | LEU | PRO | ||||
10 | THR | TRP | GLY | LEU | ALA | PRO | SER | GLY | LYS | GLY | ||||
11 | ARG | GLY | LEU | GLN | HIS | PRO | PRO | PRO | VAL | GLY | ||||
12 | SER | GLN | GLU | GLY | PRO | LYS | ASP | PRO | GLU | ALA | ||||
13 | TRP | SER | ARG | PRO | GLN | GLY | TRP | TYR | ASN | HIS | ||||
14 | SER | GLY | LYS |
sample_1: C-terminal domain of RIPK3, [U-100% 13C; U-100% 15N], 18 uM; C-terminal domain of RIPK3, [U-100% 13C; U-100% 15N], 180 uM
sample_conditions_1: ionic strength: 0 M; pH: 4; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HNHA gp | sample_1 | isotropic | sample_conditions_1 |
HSQC T1 etf 3gpsi | sample_1 | isotropic | sample_conditions_1 |
HSQC T ref 3gpsi | sample_1 | isotropic | sample_conditions_1 |
NMRFAM-SPARKY - chemical shift assignment
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks