BMRB Entry 51172

Name: Backbone NMR assignments of the chicken TRPV4 intrinsically disordered N-terminus
Published: 2022-05-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51172

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone NMR assignments of the chicken TRPV4 intrinsically disordered N-terminus

Deposition date:

2021-11-09

Original release date:

2022-05-05

Authors:

Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute

Citation:

Citation: Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute. "Backbone NMR assignments of the extensive human and chicken TRPV4 N-terminal intrinsically disordered regions as important players in ion channel regulation" Biomol. NMR Assignments 16, 205-212 (2022).
PubMed: 35451798

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ADPEDPRDAGDVLGDDSFPL SSLANLFEVEDTPSPAEPSR GPPGAVDGKQNLRMKFHGAF RKGPPKPMELLESTIYESSV VPAPKKAPMDSLFDYGTYRQ HPSENKRWRRRVVEKPVAGT KGPAPNPPPVLKV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	377
15N chemical shifts	111
1H chemical shifts	451

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IDR	1

Entities:

Entity 1, IDR 133 residues - Formula weight is not available

Residues 2-134 of the chicken TRPV4 channel (UniProtKB - A0A1D5PXA5)

1

ALA

ASP

PRO

GLU

ASP

PRO

ARG

ASP

ALA

GLY

2

ASP

VAL

LEU

GLY

ASP

SER

PHE

PRO

LEU

3

SER

LEU

ALA

ASN

LEU

PHE

GLU

VAL

GLU

4

ASP

THR

PRO

SER

PRO

ALA

GLU

PRO

SER

ARG

5

GLY

PRO

GLY

ALA

VAL

ASP

GLY

LYS

GLN

6

ASN

LEU

ARG

MET

LYS

PHE

HIS

GLY

ALA

PHE

7

ARG

LYS

GLY

PRO

LYS

PRO

MET

GLU

LEU

8

LEU

GLU

SER

THR

ILE

TYR

GLU

SER

VAL

9

VAL

PRO

ALA

PRO

LYS

ALA

PRO

MET

ASP

10

SER

LEU

PHE

ASP

TYR

GLY

THR

TYR

ARG

GLN

11

HIS

PRO

SER

GLU

ASN

LYS

ARG

TRP

ARG

12

ARG

VAL

GLU

LYS

PRO

VAL

ALA

GLY

THR

13

LYS

GLY

PRO

ALA

PRO

ASN

PRO

VAL

14

LEU

LYS

VAL

Samples:

sample_1: TRPV4-IDR, [U-13C; U-15N], 200 uM; DSS 100 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1.4 - chemical shift assignment

TOPSPIN v4.1 - processing

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz