BMRB Entry 51172

Title:
Backbone NMR assignments of the chicken TRPV4 intrinsically disordered N-terminus   PubMed: 35451798
Deposition date:
2021-11-09
Original release date:
2022-05-05
Authors:
Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute
Citation:

Citation: Goretzki, Benedikt; Tebbe, Frederike; Mitrovic, Sarah-Ana; Hellmich, Ute. "Backbone NMR assignments of the extensive human and chicken TRPV4 N-terminal intrinsically disordered regions as important players in ion channel regulation"  Biomol. NMR Assignments 16, 205-212 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Experimental source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts111
1H chemical shifts451

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IDR1

Entities:

Entity 1, IDR 133 residues - Formula weight is not available

Residues 2-134 of the chicken TRPV4 channel (UniProtKB - A0A1D5PXA5)

1   ALAASPPROGLUASPPROARGASPALAGLY
2   ASPVALLEUGLYASPASPSERPHEPROLEU
3   SERSERLEUALAASNLEUPHEGLUVALGLU
4   ASPTHRPROSERPROALAGLUPROSERARG
5   GLYPROPROGLYALAVALASPGLYLYSGLN
6   ASNLEUARGMETLYSPHEHISGLYALAPHE
7   ARGLYSGLYPROPROLYSPROMETGLULEU
8   LEUGLUSERTHRILETYRGLUSERSERVAL
9   VALPROALAPROLYSLYSALAPROMETASP
10   SERLEUPHEASPTYRGLYTHRTYRARGGLN
11   HISPROSERGLUASNLYSARGTRPARGARG
12   ARGVALVALGLULYSPROVALALAGLYTHR
13   LYSGLYPROALAPROASNPROPROPROVAL
14   LEULYSVAL

Samples:

sample_1: TRPV4-IDR, [U-13C; U-15N], 200 uM; DSS 100 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CARA v1.9.1.4 - chemical shift assignment

TOPSPIN v4.1 - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

UniProtKB A0A1D5PXA5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks