BMRB Entry 51164

Title:
Assignment of tRNAIle from Escherichia coli
Deposition date:
2021-11-01
Original release date:
2022-01-23
Authors:
de Jesus, Vanessa; Biedenbander, Thomas; Vogele, Jennifer; Wohnert, Jens; Furtig, Boris
Citation:

Citation: de Jesus, Vanessa; Biedenbander, Thomas; Vogele, Jennifer; Wohnert, Jens; Furtig, Boris. "NMR assignment of non-modified tRNA Ile from Escherichia coli"  Biomol. NMR Assign. 16, 165-170 (2022).
PubMed: 35275364

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts11
15N chemical shifts74
1H chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tRNAIle1

Entities:

Entity 1, tRNAIle 77 residues - Formula weight is not available

1   AGGCUUGUAG
2   CUCAGGUGGU
3   UAGAGCGCAC
4   CCCUGAUAAG
5   GGUGAGGUCG
6   GUGGUUCAAG
7   UCCACUCAGG
8   CCUACCA

Samples:

sample_1: tRNAIle, [U-100% 13C; U-100% 15N], 800 mM; D2O, [U-2H], 7%; DSS 250 mM; potassium chloride 200 mM; potassium phosphate 25 mM; magnesium chloride 5 mM

sample_conditions_1: ionic strength: 230 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 230 mM; pH: 6.2; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H,15N HNN-COSYsample_1isotropicsample_conditions_1
2D 1H,15N BEST-TROSYsample_1isotropicsample_conditions_1
2D 1H,1H NOESYsample_1isotropicsample_conditions_1
2D 1H,1H NOESYsample_1isotropicsample_conditions_3
2D 1H,15N CPMG NOESYsample_1isotropicsample_conditions_1
2D 1H,15N 2J SOFAST HMQCsample_1isotropicsample_conditions_1
13C-detected 2D (H)CN-HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlysample_1isotropicsample_conditions_1
2D 1H,1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.47 - chemical shift assignment

TOPSPIN v3.5 - processing

NMR spectrometers:

  • Bruker AVANCE IIIHD 600 MHz
  • Bruker AVANCE IIIHD 700 MHz
  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE IIIHD 800 MHz
  • Bruker AVANCE NEO 900 MHz
  • Bruker AVANCE NEO 950 MHz