BMRB Entry 51162

Name: N88R CtRNHI
Published: 2022-03-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51162

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N88R CtRNHI

Deposition date:

2021-10-30

Original release date:

2022-03-28

Authors:

Martin, James; Palmer, Arthur

Citation:

Citation: Martin, James; Palmer, Arthur. "Comparisons of Ribonuclease HI Homologs and Mutants Uncover a Multistate Model for Substrate Recognition" J. Am. Chem. Soc. 144, 5342-5349 (2022).
PubMed: 35312304

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Clostridium tepidum Taxonomy ID: 1962263 Superkingdom: Bacteria Kingdom: not available Genus/species: Clostridium tepidum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-25b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEKTITIYTDGAASGNPGKG GWGALLMYGSSRKEISGYDP ATTNNRMELMAAIKGLEALK EPARVQLYSDSAYLVNAMNE GWLKRWVKRGWKTAAKKPVE NIDLWQEILKLTTLHRVTFH KVKGHSDNPYNSRADELARL AIKENS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	145
1H chemical shifts	145

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N88R CtRNHI	1

Entities:

Entity 1, N88R CtRNHI 146 residues - Formula weight is not available

1

MET

GLU

LYS

THR

ILE

THR

ILE

TYR

THR

ASP

2

GLY

ALA

SER

GLY

ASN

PRO

GLY

LYS

GLY

3

GLY

TRP

GLY

ALA

LEU

MET

TYR

GLY

SER

4

SER

ARG

LYS

GLU

ILE

SER

GLY

TYR

ASP

PRO

5

ALA

THR

ASN

ARG

MET

GLU

LEU

MET

6

ALA

ILE

LYS

GLY

LEU

GLU

ALA

LEU

LYS

7

GLU

PRO

ALA

ARG

VAL

GLN

LEU

TYR

SER

ASP

8

SER

ALA

TYR

LEU

VAL

ASN

ALA

MET

ASN

GLU

9

GLY

TRP

LEU

LYS

ARG

TRP

VAL

LYS

ARG

GLY

10

TRP

LYS

THR

ALA

LYS

PRO

VAL

GLU

11

ASN

ILE

ASP

LEU

TRP

GLN

GLU

ILE

LEU

LYS

12

LEU

THR

LEU

HIS

ARG

VAL

THR

PHE

HIS

13

LYS

VAL

LYS

GLY

HIS

SER

ASP

ASN

PRO

TYR

14

ASN

SER

ARG

ALA

ASP

GLU

LEU

ALA

ARG

LEU

15

ALA

ILE

LYS

GLU

ASN

SER

Samples:

sample_1: V98A E. coli Ribonuclease HI, [U-100% 13C; U-100% 15N], 325 uM; deuterated sodium acetate 50 mM; sodium chloride 50 mM; sodium azide 0.02 % (w/v); DSS 3 mM

sample_conditions_1: ionic strength: 0.050 M; pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks