BMRB Entry 51161

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51161

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Title:
Backbone assignments of p65 DNA binding domain in complex with DNA.
Deposition date:
2021-10-29
Original release date:
2021-11-04
Authors:
Singh, Amrinder; Martinez-Yamout, Maria; Wright, Peter; Dyson, Jane
Citation:

Citation: Singh, Amrinder; Martinez-Yamout, Maria; Wright, Peter; Dyson, Jane. "Interactions of a Long Noncoding RNA with Domains of NF-kB and IkBa: Implications for the Inhibition of Non-Signal-Related Phosphorylation"  Biochemistry 61, 367-376 (2022).
PubMed: 35171557

Assembly members:

Assembly members:
entity_1, polymer, 173 residues, Formula weight is not available
entity_2, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AYVEIIEQPKQRGMRFRYKC EGRSAGSIPGERSTDTTKTH PTIKINGYTGPGTVRISLVT KDPPHRPHPHELVGKDCRDG YYEADLCPDRSIHSFQNLGI QCVKKRDLEQAISQRIQTNN NPFHVPIEEQRGDYDLNAVR LCFQVTVRDPAGRPLLLTPV LSHPIFDNRAPNT
entity_2: ATCTGGGAATTCCCAGAT

Data sets:
Data typeCount
15N chemical shifts134
1H chemical shifts134

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1p65 DBD1
2DNA chain 12
3DNA chain 22

Entities:

Entity 1, p65 DBD 173 residues - Formula weight is not available

The sequence starts from 19.

1   ALATYRVALGLUILEILEGLUGLNPROLYS
2   GLNARGGLYMETARGPHEARGTYRLYSCYS
3   GLUGLYARGSERALAGLYSERILEPROGLY
4   GLUARGSERTHRASPTHRTHRLYSTHRHIS
5   PROTHRILELYSILEASNGLYTYRTHRGLY
6   PROGLYTHRVALARGILESERLEUVALTHR
7   LYSASPPROPROHISARGPROHISPROHIS
8   GLULEUVALGLYLYSASPCYSARGASPGLY
9   TYRTYRGLUALAASPLEUCYSPROASPARG
10   SERILEHISSERPHEGLNASNLEUGLYILE
11   GLNCYSVALLYSLYSARGASPLEUGLUGLN
12   ALAILESERGLNARGILEGLNTHRASNASN
13   ASNPROPHEHISVALPROILEGLUGLUGLN
14   ARGGLYASPTYRASPLEUASNALAVALARG
15   LEUCYSPHEGLNVALTHRVALARGASPPRO
16   ALAGLYARGPROLEULEULEUTHRPROVAL
17   LEUSERHISPROILEPHEASPASNARGALA
18   PROASNTHR

Entity 2, DNA chain 1 18 residues - Formula weight is not available

1   DADTDCDTDGDGDGDADADT
2   DTDCDCDCDADGDADT

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks