BMRB Entry 5116
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5116
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Cort, J.; Mariappan, S.V.; Kim, C.; Park, M.; Peat, T.; Waldo, G.; Terwilliger, T.; Kennedy, M.. "Solution Structure of Pyrobaculum aerophilum DsrC, an archaeal homologue of
the gamma subunit of dissimilatory sulfite reductase" Eur. J. Biochem. 268, 5842-5850 (2001).
PubMed: 11722571
Assembly members:
gamma subunit of dissimilatory sulfite reductase, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 13773 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrobaculum aerophilum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
gamma subunit of dissimilatory sulfite reductase: MPVKCPGEYQVDGKKVILDE
DCFMQNPEDWDEKVAEWLAR
ELEGIQKMTEEHWKLVKYLR
EYWETFGTCPPIKMVTKETG
FSLEKIYQLFPSGPAHGACK
VAGAPKPTGCVGSHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 91 |
| 13C chemical shifts | 173 |
| 15N chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DsrC | 1 |
Entities:
Entity 1, DsrC 119 residues - Formula weight is not available
| 1 | MET | PRO | VAL | LYS | CYS | PRO | GLY | GLU | TYR | GLN | ||||
| 2 | VAL | ASP | GLY | LYS | LYS | VAL | ILE | LEU | ASP | GLU | ||||
| 3 | ASP | CYS | PHE | MET | GLN | ASN | PRO | GLU | ASP | TRP | ||||
| 4 | ASP | GLU | LYS | VAL | ALA | GLU | TRP | LEU | ALA | ARG | ||||
| 5 | GLU | LEU | GLU | GLY | ILE | GLN | LYS | MET | THR | GLU | ||||
| 6 | GLU | HIS | TRP | LYS | LEU | VAL | LYS | TYR | LEU | ARG | ||||
| 7 | GLU | TYR | TRP | GLU | THR | PHE | GLY | THR | CYS | PRO | ||||
| 8 | PRO | ILE | LYS | MET | VAL | THR | LYS | GLU | THR | GLY | ||||
| 9 | PHE | SER | LEU | GLU | LYS | ILE | TYR | GLN | LEU | PHE | ||||
| 10 | PRO | SER | GLY | PRO | ALA | HIS | GLY | ALA | CYS | LYS | ||||
| 11 | VAL | ALA | GLY | ALA | PRO | LYS | PRO | THR | GLY | CYS | ||||
| 12 | VAL | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
13C-15N_DsrC_reduced: gamma subunit of dissimilatory sulfite reductase, [U-99% 13C; U-99% 15N], 1.5 – 2.0 mM; DTT 10 mM; sodium phosphate 25 mM; sodium chloride 100 mM
sample_cond_1: pH: 7.4 na; temperature: 298 K; ionic strength: 0.125 M; pressure: 1 atm
sample_cond_2: pH*: 7.0 na; temperature: 298 K; ionic strength: 0.125 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4D 13C-separated NOESY | not available | not available | not available |
| 3D 13C,15N-simultaneous NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 1H-15N | not available | not available | not available |
| HSQC D2O exchange | not available | not available | not available |
| CBCACONNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCC-TOCSY-NNH | not available | not available | not available |
| CCC-TOCSY-NNH | not available | not available | not available |
| CBCACOCAHA | not available | not available | not available |
| 13C-HSQC | not available | not available | not available |
Software:
X-PLOR v3.84 - structure solution
FELIX v98 - processing
VNMR - collection
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks