BMRB Entry 51139

Title:
ASCC2 CUE domain interaction with K63-linked diubiquitin   PubMed: 34971705
Deposition date:
2021-10-14
Original release date:
2021-10-25
Authors:
Lombardi, Patrick; Anoh, Rita; Burke, Kate; Schmelyun, Dhane; Majumdar, Ananya; Wolberger, Cynthia
Citation:

Citation: Lombardi, Patrick; Haile, Sara; Rusanov, Timur; Rodell, Rebecca; Anoh, Rita; Baer, Julia; Burke, Kate; Gray, Lauren; Hacker, Abigail; Kebreau, Kayla; Ngandu, Christine; Orland, Hannah; Osei-Asante, Emmanuella; Schmelyun, Dhane; Shorb, Devin; Syed, Shaheer; Veilleux, Julianna; Majumdar, Ananya; Mosammaparast, Nima; Wolberger, Cynthia. "The ASCC2 CUE domain in the ALKBH3-ASCC DNA repair complex recognizes adjacent ubiquitins in K63-linked polyubiquitin"  J. Biol. Chem. 298, 101545-101545 (2022).

Assembly members:

Assembly members:
entity_1, polymer, 55 residues, Formula weight is not available
entity_2, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX-HTa

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX-HTa

Data sets:
Data typeCount
15N chemical shifts50
1H chemical shifts50
molecule interaction chemical shift values50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASCC2 CUE domain1
2K63-linked diubiquitin2

Entities:

Entity 1, ASCC2 CUE domain 55 residues - Formula weight is not available

1   GLULEUASPSERLEUILESERGLNVALLYS
2   ASPLEULEUPROASPLEUGLYGLUGLYPHE
3   ILELEUALACYSLEUGLUTYRTYRHISTYR
4   ASPPROGLUGLNVALILEASNASNILELEU
5   GLUGLUARGLEUALAPROTHRLEUSERGLN
6   LEUASPARGASNLEU

Entity 2, K63-linked diubiquitin 153 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYARGGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNARGGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLYMETGLNILEPHE
9   VALLYSTHRLEUTHRGLYLYSTHRILETHR
10   LEUGLUVALGLUPROSERASPTHRILEGLU
11   ASNVALLYSALALYSILEGLNASPLYSGLU
12   GLYILEPROPROASPGLNGLNARGLEUILE
13   PHEALAGLYLYSGLNLEUGLUASPGLYARG
14   THRLEUSERASPTYRASNILEGLNLYSGLU
15   SERTHRLEUHISLEUVALLEUARGLEUARG
16   GLYGLYASP

Samples:

sample_1: ASCC2 CUE domain, [U-15N], 20 uM; K63-linked diubiquitin 40 uM; Tris 20 mM; NaCl 100 mM; TCEP 200 mM

sample_2: ASCC2 CUE domain, [U-15N], 20 uM; K63-linked diubiquitin 20 uM; Tris 20 mM; NaCl 100 mM; TCEP 200 mM

sample_3: ASCC2 CUE domain, [U-15N], 20 uM; K63-linked diubiquitin 6 uM; Tris 20 mM; NaCl 100 mM; TCEP 200 mM

sample_4: ASCC2 CUE domain, [U-15N], 20 uM; Tris 20 mM; NaCl 100 mM; TCEP 200 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment, chemical shift calculation, peak picking

NMRPipe - processing

NMR spectrometers:

  • Bruker Ascend 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks