BMRB Entry 51134

Title:
pre-miRNA-31 BottomB
Deposition date:
2021-10-12
Original release date:
2022-02-18
Authors:
Kotar, Anita; Keane, Sarah
Citation:

Citation: Kotar, Anita; Ma, Sicong; Keane, Sarah. "pH dependence of C*A, G*A and A*A mismatches in the stem of precursor microRNA-31"  Biophys. Chem. 283, 106763-106763 (2022).
PubMed: 35114594

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: in vitro enzymatic synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCAAGAUGCUGGGAGACCA ACAUAUUGCC

Data sets:
Data typeCount
13C chemical shifts38
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA1

Entities:

Entity 1, RNA 30 residues - Formula weight is not available

1   GGCAAGAUGC
2   UGGGAGACCA
3   ACAUAUUGCC

Samples:

sample_1: RNA 0.4 mM; D2O 100%; potassium phosphate 50 mM; magnesium chloride 1 mM

sample_conditions_1: ionic strength: 0.2255 M; pH: 7.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1

Software:

NMRViewJ v9.2.0-b24 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz
  • Bruker AVANCE NEO 600 MHz