BMRB Entry 51133

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for Stress Granule Key Component G3BP1 RRM domain
Deposition date:
2021-10-12
Original release date:
2022-02-18
Authors:
Zhang, Hanyu; Tu, Xiaoming; Zhang, Jiahai
Citation:

Citation: Zhang, Hanyu; Tu, Xiaoming; Zhang, Jiahai. "1H, 13C and 15N resonance assignments of stress granule key component G3BP1 RRM domain"  Biomol. NMR Assignments 16, 109-111 (2022).
PubMed: 35150414

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28A

Data sets:
Data typeCount
13C chemical shifts265
15N chemical shifts92
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1G3BP1 RRM1

Entities:

Entity 1, G3BP1 RRM 118 residues - Formula weight is not available

The first 20 amino acids are His-Tag and the last 98 are RRM Domian.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYGLUASNLEUTYRPHEGLNHISMET
3   ASPILEGLUPROARGARGMETVALARGHIS
4   PROASPSERHISGLNLEUPHEILEGLYASN
5   LEUPROHISGLUVALASPLYSSERGLULEU
6   LYSASPPHEPHEGLNSERTYRGLYASNVAL
7   VALGLULEUARGILEASNSERGLYGLYLYS
8   LEUPROASNPHEGLYPHEVALVALPHEASP
9   ASPSERGLUPROVALGLNLYSVALLEUSER
10   ASNARGPROILEMETPHEARGGLYGLUVAL
11   ARGLEUASNVALGLUGLULYSLYSTHRARG
12   ALAALAARGGLUGLYASPARGARG

Samples:

sample_1: D2O 10%; sodium phosphate 0.02 M; sodium chloride 0.15 M; G3BP1 RRM domain, [U-99% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.27 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HACANHsample_1isotropicsample_conditions_1
3D HACACONHsample_1isotropicsample_conditions_1

Software:

SPARKY - peak picking

CARA - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Agilent INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks