BMRB Entry 51133

Name: Backbone 1H, 13C and 15N Chemical Shift Assignments for Stress Granule Key Component G3BP1 RRM domain
Published: 2022-02-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51133

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N Chemical Shift Assignments for Stress Granule Key Component G3BP1 RRM domain

Deposition date:

2021-10-12

Original release date:

2022-02-18

Authors:

Zhang, Hanyu; Tu, Xiaoming; Zhang, Jiahai

Citation:

Citation: Zhang, Hanyu; Tu, Xiaoming; Zhang, Jiahai. "1H, 13C and 15N resonance assignments of stress granule key component G3BP1 RRM domain" Biomol. NMR Assignments 16, 109-111 (2022).
PubMed: 35150414

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSHHHHHHSSGENLYFQHM DIEPRRMVRHPDSHQLFIGN LPHEVDKSELKDFFQSYGNV VELRINSGGKLPNFGFVVFD DSEPVQKVLSNRPIMFRGEV RLNVEEKKTRAAREGDRR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	265
15N chemical shifts	92
1H chemical shifts	183

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	G3BP1 RRM	1

Entities:

Entity 1, G3BP1 RRM 118 residues - Formula weight is not available

The first 20 amino acids are His-Tag and the last 98 are RRM Domian.

1

GLY

SER

HIS

SER

2

SER

GLY

GLU

ASN

LEU

TYR

PHE

GLN

HIS

MET

3

ASP

ILE

GLU

PRO

ARG

MET

VAL

ARG

HIS

4

PRO

ASP

SER

HIS

GLN

LEU

PHE

ILE

GLY

ASN

5

LEU

PRO

HIS

GLU

VAL

ASP

LYS

SER

GLU

LEU

6

LYS

ASP

PHE

GLN

SER

TYR

GLY

ASN

VAL

7

VAL

GLU

LEU

ARG

ILE

ASN

SER

GLY

LYS

8

LEU

PRO

ASN

PHE

GLY

PHE

VAL

PHE

ASP

9

ASP

SER

GLU

PRO

VAL

GLN

LYS

VAL

LEU

SER

10

ASN

ARG

PRO

ILE

MET

PHE

ARG

GLY

GLU

VAL

11

ARG

LEU

ASN

VAL

GLU

LYS

THR

ARG

12

ALA

ARG

GLU

GLY

ASP

ARG

Samples:

sample_1: D2O 10%; sodium phosphate 0.02 M; sodium chloride 0.15 M; G3BP1 RRM domain, [U-99% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.27 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HACANH	sample_1	isotropic	sample_conditions_1
3D HACACONH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - peak picking

CARA - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Agilent INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks