BMRB Entry 51132

Title:
1H, 15N and 13C sequence-specific backbone assignment of RRP1B PP1 binding domain
Deposition date:
2021-10-11
Original release date:
2022-12-20
Authors:
Bajaj, Rakhi; Senthil Kumar, Ganesan; Page, Rebecca; Peti, Wolfgang
Citation:

Citation: Srivastava, Gautam; Bajaj, Rakhi; Kumar, Ganesan Senthil; Gaudreau-Lapierre, Antoine; Nicolas, Hannah; Chamousset, Delphine; Kreitler, Dale; Peti, Wolfgang; Trinkle-Mulcahy, Laura; Page, Rebecca. "The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs"  Cell Rep. 41, 111726-111726 (2022).
PubMed: 36450254

Assembly members:

Assembly members:
entity_1, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: RP1B

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRP1B1

Entities:

Entity 1, RRP1B 105 residues - Formula weight is not available

GHM cloning artefact

1   GLYHISMETARGARGALALYSSERSERTHR
2   ALATHRHISPROPROGLYPROALAVALGLN
3   LEUASNLYSTHRPROSERSERSERLYSLYS
4   VALTHRPHEGLYLEUASNARGASNMETTHR
5   ALAGLUPHELYSLYSTHRASPLYSSERILE
6   LEUVALSERPROTHRGLYPROSERARGVAL
7   ALAPHEASPPROGLUGLNLYSPROLEUHIS
8   GLYVALLEULYSTHRPROTHRSERSERPRO
9   ALASERSERPROLEUVALALALYSLYSPRO
10   LEUTHRTHRTHRPROARGARGARGPROARG
11   ALAMETASPPHEPHE

Samples:

sample_1: RRP1B, [U-99% 13C; U-99% 15N], 0.6 mM; RRP1B, [U-99% 15N], 0.6 mM; Bis-Tris 20 mM; NaCl 0.15 M; TCEP 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

TOPSPIN - collection, processing

NMR spectrometers:

  • Bruker AVANCE III 850 MHz
  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks