BMRB Entry 51131

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51131

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Title:
Resonance assignment of the isoleucine delta1 methyl groups for beta-arrestin 1 cysteine-less mutant
Deposition date:
2021-10-11
Original release date:
2021-12-07
Authors:
Shiraishi, Yutaro; Shimada, Ichio
Citation:

Citation: Shiraishi, Yutaro; Kofuku, Yutaka; Ueda, Takumi; Pandey, Shubhi; Dwivedi-Agnihotri, Hemlata; Shukla, Arun; Shimada, Ichio. "Biphasic activation of beta-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR"  Nat. Commun. 12, 7158-7158 (2021).
PubMed: 34887409

Assembly members:

Assembly members:
entity_1, polymer, 419 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrcHisB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMGDKGTRVFKKASPNGKLT VYLGKRDFVDHIDLVDPVDG VVLVDPEYLKERRVYVTLTV AFRYGREDLDVLGLTFRKDL FVANVQSFPPAPEDKKPLTR LQERLIKKLGEHAYPFTFEI PPNLPSSVTLQPGPEDTGKA LGVDYEVKAFVAENLEEKIH KRNSVRLVIRKVQYAPERPG PQPTAETTRQFLMSDKPLHL EASLDKEIYYHGEPISVNVH VTNNTNKTVKKIKISVRQYA DIVLFNTAQYKVPVAMEEAD DTVAPSSTFSKVYTLTPFLA NNREKRGLALDGKLKHEDTN LASSTLLREGANREILGIIV SYKVKVKLVVSRGGLLGDLA SSDVAVELPFTLMHPKPKEE PPHREVPENETPVDTNLIEL DTNDDDIVFEDFARQRLKGM KDDKEEEEDGTGSPQLNNR

Data sets:
Data typeCount
13C chemical shifts15
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1beta-arrestin 1 cyctsine-less mutant1

Entities:

Entity 1, beta-arrestin 1 cyctsine-less mutant 419 residues - Formula weight is not available

1   GLYMETGLYASPLYSGLYTHRARGVALPHE
2   LYSLYSALASERPROASNGLYLYSLEUTHR
3   VALTYRLEUGLYLYSARGASPPHEVALASP
4   HISILEASPLEUVALASPPROVALASPGLY
5   VALVALLEUVALASPPROGLUTYRLEULYS
6   GLUARGARGVALTYRVALTHRLEUTHRVAL
7   ALAPHEARGTYRGLYARGGLUASPLEUASP
8   VALLEUGLYLEUTHRPHEARGLYSASPLEU
9   PHEVALALAASNVALGLNSERPHEPROPRO
10   ALAPROGLUASPLYSLYSPROLEUTHRARG
11   LEUGLNGLUARGLEUILELYSLYSLEUGLY
12   GLUHISALATYRPROPHETHRPHEGLUILE
13   PROPROASNLEUPROSERSERVALTHRLEU
14   GLNPROGLYPROGLUASPTHRGLYLYSALA
15   LEUGLYVALASPTYRGLUVALLYSALAPHE
16   VALALAGLUASNLEUGLUGLULYSILEHIS
17   LYSARGASNSERVALARGLEUVALILEARG
18   LYSVALGLNTYRALAPROGLUARGPROGLY
19   PROGLNPROTHRALAGLUTHRTHRARGGLN
20   PHELEUMETSERASPLYSPROLEUHISLEU
21   GLUALASERLEUASPLYSGLUILETYRTYR
22   HISGLYGLUPROILESERVALASNVALHIS
23   VALTHRASNASNTHRASNLYSTHRVALLYS
24   LYSILELYSILESERVALARGGLNTYRALA
25   ASPILEVALLEUPHEASNTHRALAGLNTYR
26   LYSVALPROVALALAMETGLUGLUALAASP
27   ASPTHRVALALAPROSERSERTHRPHESER
28   LYSVALTYRTHRLEUTHRPROPHELEUALA
29   ASNASNARGGLULYSARGGLYLEUALALEU
30   ASPGLYLYSLEULYSHISGLUASPTHRASN
31   LEUALASERSERTHRLEULEUARGGLUGLY
32   ALAASNARGGLUILELEUGLYILEILEVAL
33   SERTYRLYSVALLYSVALLYSLEUVALVAL
34   SERARGGLYGLYLEULEUGLYASPLEUALA
35   SERSERASPVALALAVALGLULEUPROPHE
36   THRLEUMETHISPROLYSPROLYSGLUGLU
37   PROPROHISARGGLUVALPROGLUASNGLU
38   THRPROVALASPTHRASNLEUILEGLULEU
39   ASPTHRASNASPASPASPILEVALPHEGLU
40   ASPPHEALAARGGLNARGLEULYSGLYMET
41   LYSASPASPLYSGLUGLUGLUGLUASPGLY
42   THRGLYSERPROGLNLEUASNASNARG

Samples:

sample_1: beta-arrestin 1 cycteine-less mutant, [U-2H; 13CD,1HD-Ile], 15 uM; HEPES 20 mM; sodium chloride 150 mM; D2O, [U-2H], 100%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC/HMQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - processing

SPARKY - peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz