Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51130
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Citation: Lombardi, Patrick; Haile, Sara; Rusanov, Timur; Rodell, Rebecca; Anoh, Rita; Baer, Julia; Burke, Kate; Gray, Lauren; Hacker, Abigail; Kebreau, Kayla; Ngandu, Christine; Orland, Hannah; Osei-Asante, Emmanuella; Schmelyun, Dhane; Shorb, Devin; Syed, Shaheer; Veilleux, Julianna; Majumdar, Ananya; Mosammaparast, Nima; Wolberger, Cynthia. "The ASCC2 CUE domain in the ALKBH3-ASCC DNA repair complex recognizes adjacent ubiquitins in K63-linked polyubiquitin" J. Biol. Chem. 298, 101545-101545 (2022).
PubMed: 34971705
Assembly members:
entity_1, polymer, 55 residues, Formula weight is not available
entity_2, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPROEX-HTa
Entity Sequences (FASTA):
entity_1: ELDSLISQVKDLLPDLGEGF
ILACLEYYHYDPEQVINNIL
EERLAPTLSQLDRNL
entity_2: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGRQLEDGRTLSDYN
IQRESTLHLVLRLRGG
Data type | Count |
15N chemical shifts | 50 |
1H chemical shifts | 50 |
molecule interaction chemical shift values | 50 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ASCC2 CUE domain | 1 |
2 | monoubiquitin | 2 |
Entity 1, ASCC2 CUE domain 55 residues - Formula weight is not available
1 | GLU | LEU | ASP | SER | LEU | ILE | SER | GLN | VAL | LYS | ||||
2 | ASP | LEU | LEU | PRO | ASP | LEU | GLY | GLU | GLY | PHE | ||||
3 | ILE | LEU | ALA | CYS | LEU | GLU | TYR | TYR | HIS | TYR | ||||
4 | ASP | PRO | GLU | GLN | VAL | ILE | ASN | ASN | ILE | LEU | ||||
5 | GLU | GLU | ARG | LEU | ALA | PRO | THR | LEU | SER | GLN | ||||
6 | LEU | ASP | ARG | ASN | LEU |
Entity 2, monoubiquitin 76 residues - Formula weight is not available
1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | ARG | GLN | LEU | ||||
6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
7 | ILE | GLN | ARG | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
8 | LEU | ARG | LEU | ARG | GLY | GLY |
sample_1: ASCC2 CUE domain, [U-15N], 20 uM; monoubiquitin 681 uM; Tris 20 mM; NaCl 100 mM; TCEP 200 mM
sample_2: ASCC2 CUE domain, [U-15N], 20 uM; Tris 20 mM; NaCl 100 mM; TCEP 200 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; temperature: 313 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
CcpNMR - chemical shift assignment, chemical shift calculation, peak picking
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