BMRB Entry 51121

Title:
Single residue switches of aggregation in the N-terminal 'master controller' region of alpha-synuclein
Deposition date:
2021-10-01
Original release date:
2022-07-28
Authors:
Ulamec, Sabine; Maya-Martinez, Roberto; Radford, Sheena; Brockwell, David
Citation:

Citation: Doherty, Ciaran; Ulamec, Sabine; Maya-Martinez, Roberto; Good, Sarah; Makepeace, Jemma; Khan, G Nasir; van Oosten-Hawle, Patricija; Radford, Sheena; Brockwell, David. "A short motif in the N-terminal region of a-synuclein is critical for both aggregation and function."  Nat. Struct. Mol. Biol. 27, 249-259 (2020).
PubMed: 32157247

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, 13784.37 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts129
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human alpha-Synuclein1

Entities:

Entity 1, Human alpha-Synuclein 133 residues - 13784.37 Da.

D36-42 (P1)

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLULYSTHRLYSGLUGLY
5   VALVALHISGLYVALALATHRVALALAGLU
6   LYSTHRLYSGLUGLNVALTHRASNVALGLY
7   GLYALAVALVALTHRGLYVALTHRALAVAL
8   ALAGLNLYSTHRVALGLUGLYALAGLYSER
9   ILEALAALAALATHRGLYPHEVALLYSLYS
10   ASPGLNLEUGLYLYSASNGLUGLUGLYALA
11   PROGLNGLUGLYILELEUGLUASPMETPRO
12   VALASPPROASPASNGLUALATYRGLUMET
13   PROSERGLUGLUGLYTYRGLNASPTYRGLU
14   PROGLUALA

Samples:

sample_1: Human alpha-Synuclein (D36-42), [U-13C; U-15N], 200 uM; sodium chloride 20 mM; Tris-HCl 20 mM; Sodium Azide 0.02%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 288.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D hN(CA)NNHsample_1isotropicsample_conditions_1
3D hN(COCA)NNHsample_1isotropicsample_conditions_1
3D HNN-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe vX - processing

TOPSPIN v3.5 - collection

CCPN/Analysis vv.2.4.2 - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Ascend Aeon 950 MHz MHz

Related Database Links:

NCBI 6622
UNP P37840
PDB
AlphaFold Q6IAU6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks