BMRB Entry 51120

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51120

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Title:
Single residue switches of aggregation in the N-terminal 'master controller' region of alpha-synuclein
Deposition date:
2021-10-01
Original release date:
2022-07-28
Authors:
Ulamec, Sabine; Maya-Martinez, Roberto; Radford, Sheena; Brockwell, David
Citation:

Citation: Doherty, Ciaran; Ulamec, Sabine; Maya-Martinez, Roberto; Good, Sarah; Makepeace, Jemma; Khan, G Nasir; van Oosten-Hawle, Patricija; Radford, Sheena; Brockwell, David. "A short motif in the N-terminal region of a-synuclein is critical for both aggregation and function."  Nat. Struct. Mol. Biol. 27, 249-259 (2020).
PubMed: 32157247

Assembly members:

Assembly members:
entity_1, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts136
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human alpha-Synuclein1

Entities:

Entity 1, Human alpha-Synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: Human alpha-Synuclein, [U-13C; U-15N], 200 uM; sodium chloride 20 mM; Tris-HCl 20 mM; Sodium Azide 0.002%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 288.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)NHsample_1isotropicsample_conditions_1
3D hN(COCA)NNHsample_1isotropicsample_conditions_1
3D HNN-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe vX - processing

TOPSPIN v3.5 - collection

CCPN vv.2.4.2 - chemical shift assignment, data analysis

NMR spectrometers:

  • Oxford Oxford 750 MHz MHz

Related Database Links:

UNP P37840
NCBI 6622
AlphaFold Q6IAU6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks