BMRB Entry 51119

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51119

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Title:
Backbone relaxation rates of the fusion of ZNG peptide with the zinc finger domain of murine methionine amino peptidase 1
Deposition date:
2021-09-30
Original release date:
2022-06-01
Authors:
Edmonds, Katherine; Jordan, Matthew; Thalluri, Kishore; Hongwei, Wu; Di Marchi, Richard; Giedroc, David
Citation:

Citation: Weiss, Andy; Murdoch, Caitlin; Edmonds, Katherine; Jordan, Matthew; Monteith, Andrew; Perera, Yasiru; Rodriguez Nassif, Aslin; Petoletti, Amber; Beavers, William; Munneke, Matthew; Drury, Sydney; Krystofiak, Evan; Thalluri, Kishore; Wu, Hongwei; Kruse, Angela; DiMarchi, Richard; Caprioli, Richard; Spraggins, Jeffrey; Chazin, Walter; Giedroc, David; Skaar, Eric. "Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis"  Cell 185, 2148-2163 (2022).
PubMed: 35584702

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AEEEYAEDCPELVPIETKNQ EMAAVETRVCETDGCSSEAK LQCPTCIKLGIQGSYFCSQE CFKGSWATHKLLHKKAKDEK

Data sets:
Data typeCount
T1 relaxation values75
T2 relaxation values75
heteronuclear NOE values75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zf1
2zn12
3zn12

Entities:

Entity 1, zf 80 residues - Formula weight is not available

1   ALAGLUGLUGLUTYRALAGLUASPCYSPRO
2   GLULEUVALPROILEGLUTHRLYSASNGLN
3   GLUMETALAALAVALGLUTHRARGVALCYS
4   GLUTHRASPGLYCYSSERSERGLUALALYS
5   LEUGLNCYSPROTHRCYSILELYSLEUGLY
6   ILEGLNGLYSERTYRPHECYSSERGLNGLU
7   CYSPHELYSGLYSERTRPALATHRHISLYS
8   LEULEUHISLYSLYSALALYSASPGLULYS

Entity 2, zn1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Metap1 zinc finger, [U-100% 13C; U-100% 15N], 1 ± 0.01 mM; ZINC ION 2 ± 0.001 mM; DSS 0.3 ± 0.01 mM; sodium chloride 150 ± 0.01 mM; sodium phosphate 10 ± 0.01 mM; TCEP 2 ± 0.01 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.9 - collection

NMRFAM-SPARKY - data analysis

NMRPipe - processing

istHMS - processing

CARA - data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Related Database Links:

UNP Q8BP48 Q8VEH6
AlphaFold Q8BP48 Q8K399