BMRB Entry 51116

Name: Backbone NMR resonance assignment of the disordered UBact protein from Nitrospira nitrosa
Published: 2021-10-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51116

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone NMR resonance assignment of the disordered UBact protein from Nitrospira nitrosa

Deposition date:

2021-09-30

Original release date:

2021-10-31

Authors:

Bonn, Steven; Fushman, David

Citation:

Citation: Bonn, Steven; Fushman, David. "Backbone NMR resonance assignment of the intrinsically disordered UBact protein from Nitrospira nitrosa" Biomol. NMR Assign. 16, 129-134 (2022).
PubMed: 35107780

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Nitrospira nitrosa Taxonomy ID: 1742972 Superkingdom: Bacteria Kingdom: not available Genus/species: Nitrospira nitrosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-41a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MIQSLMPERRERPGDPMPKS PSPLEEGGGPRRPETGSPDK DSLLKRMRRVDPKQAERYRQ RTGE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	165
15N chemical shifts	59
1H chemical shifts	173

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBact	1

Entities:

Entity 1, UBact 64 residues - Formula weight is not available

1

MET

ILE

GLN

SER

LEU

MET

PRO

GLU

ARG

2

GLU

ARG

PRO

GLY

ASP

PRO

MET

PRO

LYS

SER

3

PRO

SER

PRO

LEU

GLU

GLY

PRO

4

ARG

PRO

GLU

THR

GLY

SER

PRO

ASP

LYS

5

ASP

SER

LEU

LYS

ARG

MET

ARG

VAL

6

ASP

PRO

LYS

GLN

ALA

GLU

ARG

TYR

ARG

GLN

7

ARG

THR

GLY

GLU

Samples:

sample_1: UBact, [U-98% 13C; U-98% 15N], 500 uM; sodium phosphate 20 mM; D2O 5%

sample_conditions_1: ionic strength: 36 mM; pH: 6.8; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCOCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.8 - collection, processing

CcpNMR v2.4.2 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz