BMRB Entry 51115
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51115
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Malki, Anas; Teulon, Jean-Marie; Camacho Zarco, Aldo; Chen, Shu-Wen; Adamski, Wiktor; Maurin, Damien; Salvi, Nicola; Pellequer, Jean-Luc; Blackledge, Martin. "Intrinsically Disordered Tardigrade Proteins Self-Assemble into Fibrous Gels in Response to Environmental Stress" Angew. Chem. Int. Ed. Engl. 61, e202109961-e202109961 (2022).
PubMed: 34750927
Assembly members:
entity_1, polymer, 233 residues, Formula weight is not available
Natural source: Common Name: Hypsibius exemplaris Taxonomy ID: 2072580 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hypsibius exemplaris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28b(+)
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 636 |
| 15N chemical shifts | 218 |
| 1H chemical shifts | 218 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CAHS_8 | 1 |
Entities:
Entity 1, CAHS_8 233 residues - Formula weight is not available
| 1 | MET | SER | GLY | ARG | ASN | VAL | GLU | SER | HIS | MET | ||||
| 2 | GLU | ARG | ASN | GLU | LYS | VAL | VAL | VAL | ASN | ASN | ||||
| 3 | SER | GLY | HIS | ALA | ASP | VAL | LYS | LYS | GLN | GLN | ||||
| 4 | GLN | GLN | VAL | GLU | HIS | THR | GLU | PHE | THR | HIS | ||||
| 5 | THR | GLU | VAL | LYS | ALA | PRO | LEU | ILE | HIS | PRO | ||||
| 6 | ALA | PRO | PRO | ILE | ILE | SER | THR | GLY | ALA | ALA | ||||
| 7 | GLY | LEU | ALA | GLU | GLU | ILE | VAL | GLY | GLN | GLY | ||||
| 8 | PHE | THR | ALA | SER | ALA | ALA | ARG | ILE | SER | GLY | ||||
| 9 | GLY | THR | ALA | GLU | VAL | HIS | LEU | GLN | PRO | SER | ||||
| 10 | ALA | ALA | MET | THR | GLU | GLU | ALA | ARG | ARG | ASP | ||||
| 11 | GLN | GLU | ARG | TYR | ARG | GLN | GLU | GLN | GLU | SER | ||||
| 12 | ILE | ALA | LYS | GLN | GLN | GLU | ARG | GLU | MET | GLU | ||||
| 13 | LYS | LYS | THR | GLU | ALA | TYR | ARG | LYS | THR | ALA | ||||
| 14 | GLU | ALA | GLU | ALA | GLU | LYS | ILE | ARG | LYS | GLU | ||||
| 15 | LEU | GLU | LYS | GLN | HIS | ALA | ARG | ASP | VAL | GLU | ||||
| 16 | PHE | ARG | LYS | ASP | LEU | ILE | GLU | SER | THR | ILE | ||||
| 17 | ASP | ARG | GLN | LYS | ARG | GLU | VAL | ASP | LEU | GLU | ||||
| 18 | ALA | LYS | MET | ALA | LYS | ARG | GLU | LEU | ASP | ARG | ||||
| 19 | GLU | GLY | GLN | LEU | ALA | LYS | GLU | ALA | LEU | GLU | ||||
| 20 | ARG | SER | ARG | LEU | ALA | THR | ASN | VAL | GLU | VAL | ||||
| 21 | ASN | PHE | ASP | SER | ALA | ALA | GLY | HIS | THR | VAL | ||||
| 22 | SER | GLY | GLY | THR | THR | VAL | SER | THR | SER | ASP | ||||
| 23 | LYS | MET | GLU | ILE | LYS | ARG | ASN | GLU | ASN | LEU | ||||
| 24 | TYR | PHE | GLN |
Samples:
sample_1: CAHS_8, [U-100% 13C; U-100% 15N; U-80% 2H], 350 uM; sodium phosphate 50 mM; sodium chloride 500 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.3 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks