BMRB Entry 51113

Name: The 1H, 15N, and 13C resonance assignments of the low-complexity domain from the oncogenic fusion protein EWS-FLI1
Published: 2022-02-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51113

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The 1H, 15N, and 13C resonance assignments of the low-complexity domain from the oncogenic fusion protein EWS-FLI1

Deposition date:

2021-09-29

Original release date:

2022-02-18

Authors:

Johnson, Courtney; Xu, Xiaoping; Holloway, Stephen; Libich, David

Citation:

Citation: Johnson, Courtney; Xu, Xiaoping; Holloway, Stephen; Libich, David. "The 1H, 15N and 13C resonance assignments of the low-complexity domain from the oncogenic fusion protein EWS-FLI1" Biomol. NMR Assignments 16, 67-73 (2022).
PubMed: 34994941

Assembly members:

Assembly members:
entity_1, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pAG8H-GB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TYPQVPGSYPMQPVTAPPSY PPTSYSSTQPTSYDQSSYSQ QNTYGQPSSYGQQSSYGQQS SYGQQPPTSYPPQTGSYSQA PSQYSQQSSSYGQQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	262
15N chemical shifts	78
1H chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EWSR1 171-264	1

Entities:

Entity 1, EWSR1 171-264 94 residues - Formula weight is not available

1

THR

TYR

PRO

GLN

VAL

PRO

GLY

SER

TYR

PRO

2

MET

GLN

PRO

VAL

THR

ALA

PRO

SER

TYR

3

PRO

THR

SER

TYR

SER

THR

GLN

PRO

4

THR

SER

TYR

ASP

GLN

SER

TYR

SER

GLN

5

GLN

ASN

THR

TYR

GLY

GLN

PRO

SER

TYR

6

GLY

GLN

SER

TYR

GLY

GLN

SER

7

SER

TYR

GLY

GLN

PRO

THR

SER

TYR

8

PRO

GLN

THR

GLY

SER

TYR

SER

GLN

ALA

9

PRO

SER

GLN

TYR

SER

GLN

SER

10

TYR

GLY

GLN

Samples:

sample_1: EWSR1 171-264, [U-100% 13C; U-100% 15N], 100 uM; MES 20 mM; PMSF 0.1 mM; EDTA 1 mM

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

NMRDraw - processing

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks