BMRB Entry 51110

Title:
Backbone 1H, 15N Chemical Shift Assignments for RGL1-Ras-association domain
Deposition date:
2021-09-29
Original release date:
2022-03-11
Authors:
Eves, Ben; Gebregiworgis, Teklab; Gasmi-Seabrook, Genevieve; Kuntz, Douglas; Prive, Gilbert; Marshall, Christopher; Ikura, Mitsu
Citation:

Citation: Eves, Ben; Gebregiworgis, Teklab; Gasmi-Seabrook, Genevieve; Kuntz, Douglas; Prive, Gilbert; Marshall, Christopher; Ikura, Mitsu. "Structures of RGL1 RAS-Association domain in complex with KRAS and the oncogenic G12V mutant"  J. Mol. Biol. 434, 167527-167527 (2022).
PubMed: 35257782

Assembly members:

Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEXascpac

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RGL1-Ras-association domain monomer1

Entities:

Entity 1, RGL1-Ras-association domain monomer 93 residues - Formula weight is not available

1   GLNGLNASNGLUASPTHRCYSILEILEARG
2   ILESERVALGLUASPASNASNGLYASNMET
3   TYRLYSSERILEMETLEUTHRSERGLNASP
4   LYSTHRPROALAVALILEGLNARGALAMET
5   LEULYSHISASNLEUASPSERASPPROALA
6   GLUGLUTYRGLULEUVALGLNVALILESER
7   GLUASPLYSGLULEUVALILEPROASPSER
8   ALAASNVALPHETYRALAMETASNSERGLN
9   VALASNPHEASPPHEILELEUARGLYSLYS
10   ASNSERMET

Samples:

sample_1: RGL1-Ras-association domain, [U-13C; U-15N], 200 uM; HEPES pH 7.4 20 mM; NaCl 100 mM; TCEP 2 mM; MgCl2 5 mM; sodium azide 0.01%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment, peak picking

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks