BMRB Entry 51109

Title:
1H,15N and 13C backbone chemical shift assignment of Spy29-124 in complex with Im7A3
Deposition date:
2021-09-28
Original release date:
2022-04-08
Authors:
He, Wei; Li, Xinming; Xue, Hongjuan; Yang, Yuanyuan; Mencius, Jun; Bai, Ling; Zhang, Jiayin; Wu, Bin; Xue, Yi; Quan, Shu
Citation:

Citation: He, Wei; Li, Xinming; Xue, Hongjuan; Yang, Yuanyuan; Mencius, Jun; Bai, Ling; Zhang, Jiayin; Xu, Jianhe; Wu, Bin; Xue, Yi; Quan, Shu. "Insights into the client protein release mechanism of the ATP-independent chaperone Spy"  Nat. commun. 13, 2818-2818 (2022).
PubMed: 35595811

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, Formula weight is not available
entity_2, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET28b(+)-HisSUMO-spy29-124

Data sets:
Data typeCount
13C chemical shifts260
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1spy29-1241
2Im7A32

Entities:

Entity 1, spy29-124 96 residues - Formula weight is not available

1   PHELYSASPLEUASNLEUTHRASPALAGLN
2   LYSGLNGLNILEARGGLUILEMETLYSGLY
3   GLNARGASPGLNMETLYSARGPROPROLEU
4   GLUGLUARGARGALAMETHISASPILEILE
5   ALASERASPTHRPHEASPLYSVALLYSALA
6   GLUALAGLNILEALALYSMETGLUGLUGLN
7   ARGLYSALAASNMETLEUALAHISMETGLU
8   THRGLNASNLYSILETYRASNILELEUTHR
9   PROGLUGLNLYSLYSGLNPHEASNALAASN
10   PHEGLULYSARGLEUTHR

Entity 2, Im7A3 87 residues - Formula weight is not available

1   SERGLULEULYSASNSERILESERASPTYR
2   THRGLUALAGLUPHEVALGLNALAALALYS
3   GLUILEGLULYSGLUASNVALALAALATHR
4   ASPASPVALLEUASPVALALALEUGLUHIS
5   PHEVALLYSILETHRGLUHISPROASPGLY
6   THRASPLEUILETYRTYRPROSERASPASN
7   ARGASPASPSERPROGLUGLYILEVALLYS
8   GLUILELYSGLUTRPARGALAALAASNGLY
9   LYSPROGLYPHELYSGLNGLY

Samples:

sample_1: spy1-124, [U-13C; U-15N; U-2H], 0.4 mM; HEPES 40 mM; NaCl 150 mM; DTT 3 mM; PMSF 1 mM; NaN3 0.03%; inhibitor cocktail 100 x; Im7A3 0.4 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D trHNCOsample_1isotropicsample_conditions_1
3D trHNCACOsample_1isotropicsample_conditions_1
3D trHNCOCAsample_1isotropicsample_conditions_1
3D trHNCAsample_1isotropicsample_conditions_1
3D trHN(CA)CBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe v9.7 - processing

NMRFAM-SPARKY v1.2 - data analysis

qMDD v1.2 - data processing

NMR spectrometers:

  • Agilent DD2 800 MHz
  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks