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warning: failed to read freetag - ' _nef_peak\.ccpn_peak_list_serial'
warning: failed to read freetag - ' _nef_peak\.ccpn_peak_list_serial'
warning: failed to read freetag - ' _nef_peak\.ccpn_peak_list_serial'
warning: failed to read freetag - ' _nef_peak\.ccpn_peak_list_serial'
warning: failed to read freetag - ' _nef_peak\.ccpn_peak_list_serial'
warning: failed to read freetag - ' _nef_peak\.ccpn_peak_list_serial'
BMRB Entry 51093 BMRB - Biological Magnetic Resonance Bank

BMRB Entry 51093

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments of GB1_pSu9(1-69) construct
Deposition date:
2021-09-22
Original release date:
2022-03-21
Authors:
Schanda, Paul; Sucec, Iva; Feignier, Antoine
Citation:

Citation: Schanda, Paul; Sucec, Iva; Feignier, Antoine. "Backbone 1H, 13C and 15N Chemical Shift Assignments of GB1_pSu9(1-69) construct"  .

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Neurospora crassa   Taxonomy ID: 5141   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Neurospora crassa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET_GB1_pSu9(1-69)

Data typeCount
13C chemical shifts353
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB1_pSu9(1-69)1

Entities:

Entity 1, GB1_pSu9(1-69) 149 residues - Formula weight is not available

1   HISHISHISHISHISHISPROMETLYSGLN
2   TYRLYSLEUILELEUASNGLYLYSTHRLEU
3   LYSGLYGLUTHRTHRTHRGLUALAVALASP
4   ALAALATHRALAGLULYSVALPHELYSGLN
5   TYRALAASNASPASNGLYVALASPGLYGLU
6   TRPTHRTYRASPASPALATHRLYSTHRPHE
7   THRVALTHRGLUGLYSERGLYSERGLYSER
8   GLUASNLEUTYRPHEGLNGLYHISMETALA
9   SERTHRARGVALLEUALASERARGLEUALA
10   SERGLNMETALAALASERALALYSVALALA
11   ARGPROALAVALARGVALALAGLNVALSER
12   LYSARGTHRILEGLNTHRGLYSERPROLEU
13   GLNTHRLEULYSARGTHRGLNMETTHRSER
14   ILEVALASNALATHRTHRARGGLNALAPHE
15   GLNLYSARGALATYRSERSERLEUGLU

Samples:

sample_1: GB1_pSu9(1-69), [U-100% 13C; U-100% 15N], 698 uM; H2O 55.5 M; D2O 10%; sodium chloride 100 mM; TRIS 20 mM

sample_conditions_1: pH: 7; temperature: 293.15 K

sample_conditions_2: pH: 7; temperature: 298.15 K

sample_conditions_3: pH: 7; temperature: 300.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_3
3D HN(CO)CAsample_1isotropicsample_conditions_3
HncocaNHsample_1isotropicsample_conditions_1
HncacoNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR - chemical shift assignment, peak picking

CYANA - data analysis

TALOS+ - data analysis

NMR spectrometers:

  • Bruker Ascend 700 MHz
  • Bruker Ascend 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks