BMRB Entry 51092

Title:
Assignment of BRCA1-A complex subunit RAP80 35-124 C70S, 97-103 poly A linker
Deposition date:
2021-09-20
Original release date:
2021-10-01
Authors:
Gaussmann, Stefan; Sattler, Michael
Citation:

Citation: Fottner, Maximilian; Weyh, Maria; Gaussmann, Stefan; Schwarz, Dominic; Sattler, Michael; Lang, Kathrin. "A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes"  Nat. Commun. 12, 6515-6515 (2021).
PubMed: 34764289

Assembly members:

Assembly members:
entity_1, polymer, 102 residues, 11234.39 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-17b

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RAP801

Entities:

Entity 1, RAP80 102 residues - 11234.39 Da.

1   PROSERSERHISHISHISHISHISHISSER
2   SERGLYARGLEUGLUASPALAPHEILEVAL
3   ILESERASPSERASPGLYGLUGLUPROLYS
4   GLUGLUASNGLYLEUGLNLYSTHRLYSTHR
5   LYSGLNSERASNARGALALYSSERLEUALA
6   LYSARGLYSILEALAGLNMETTHRGLUGLU
7   GLUGLNPHEALALEUALALEULYSMETSER
8   GLUGLNGLUALAALAALAALAALAALAALA
9   ALAGLUGLUGLUGLUGLULEULEUARGLYS
10   ALAILEALAGLUSERLEUASNSERCYSARG
11   PROSER

Samples:

sample_1: BRCA1-A complex subunit RAP80, [U-99% 13C; U-99% 15N], 180 uM; TRIS 20 mM; DTT 2 mM; D2O 10%; sodium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 pl7 - collection

NMRPipe vLinux64:20210129 - processing

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Related Database Links:

SP Q96RL1
AlphaFold Q9UHX7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks