BMRB Entry 51092

Name: Assignment of BRCA1-A complex subunit RAP80 35-124 C70S, 97-103 poly A linker
Published: 2021-10-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51092

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of BRCA1-A complex subunit RAP80 35-124 C70S, 97-103 poly A linker

Deposition date:

2021-09-20

Original release date:

2021-10-01

Authors:

Gaussmann, Stefan; Sattler, Michael

Citation:

Citation: Fottner, Maximilian; Weyh, Maria; Gaussmann, Stefan; Schwarz, Dominic; Sattler, Michael; Lang, Kathrin. "A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes" Nat. Commun. 12, 6515-6515 (2021).
PubMed: 34764289

Assembly members:

Assembly members:
entity_1, polymer, 102 residues, 11234.39 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PSSHHHHHHSSGRLEDAFIV ISDSDGEEPKEENGLQKTKT KQSNRAKSLAKRKIAQMTEE EQFALALKMSEQEAAAAAAA AEEEEELLRKAIAESLNSCR PS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	338
15N chemical shifts	83
1H chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RAP80	1

Entities:

Entity 1, RAP80 102 residues - 11234.39 Da.

1

PRO

SER

HIS

SER

2

SER

GLY

ARG

LEU

GLU

ASP

ALA

PHE

ILE

VAL

3

ILE

SER

ASP

SER

ASP

GLY

GLU

PRO

LYS

4

GLU

ASN

GLY

LEU

GLN

LYS

THR

LYS

THR

5

LYS

GLN

SER

ASN

ARG

ALA

LYS

SER

LEU

ALA

6

LYS

ARG

LYS

ILE

ALA

GLN

MET

THR

GLU

7

GLU

GLN

PHE

ALA

LEU

ALA

LEU

LYS

MET

SER

8

GLU

GLN

GLU

ALA

9

ALA

GLU

LEU

ARG

LYS

10

ALA

ILE

ALA

GLU

SER

LEU

ASN

SER

CYS

ARG

11

PRO

SER

Samples:

sample_1: BRCA1-A complex subunit RAP80, [U-99% 13C; U-99% 15N], 180 uM; TRIS 20 mM; DTT 2 mM; D2O 10%; sodium chloride 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5 pl7 - collection

NMRPipe vLinux64:20210129 - processing

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 900 MHz

SP	Q96RL1
AlphaFold	Q9UHX7