BMRB Entry 51086

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Transforming Growth Factor Beta Receptor 1 (TbRI) as bound to Domain 2 of Heligmosomoides polygyrus protein Transforming Growth Factor Beta Mimic 1 (TGM-1 D2)
Published: 2021-09-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51086

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Transforming Growth Factor Beta Receptor 1 (TbRI) as bound to Domain 2 of Heligmosomoides polygyrus protein Transforming Growth Factor Beta Mimic 1 (TGM-1 D2)

Deposition date:

2021-09-14

Original release date:

2021-09-18

Authors:

Mukundan, Ananya; Byeon, Chang-Hyeock

Citation:

Citation: Mukundan, Ananya; Byeon, Chang-Hyeock; Hinck, Cynthia; Cunningham, Kyle; Campion, Tiffany; Smyth, Danielle; Maizels, Rick; Hinck, Andrew. "Convergent evolution of a parasite-encoded complement control protein-scaffold to mimic binding of mammalian TGF-b to its receptors, TbRI and TbRII" J. Biol. Chem. 298, 101994-101994 (2022).
PubMed: 35500648

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, 9451 Da.
entity_2, polymer, 83 residues, 9296 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ATALQCFCHLCTKDNFTCVT DGLCFVSVTETTDKVIHNSS CIAEIDLIPRDRPFVCAPSS KTGSVTTTYCCNQDHCNKIE LPTTV
entity_2: GTRCSPLPTNDTVSFEYLKA TVNPGIIFNITVHPDASGKY PELTYIKRICKNFPTDSNVQ GHIIGMCYNAEWQFSSTPTC PAS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	209
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TbRI	1
2	TGM-D2	2

Entities:

Entity 1, TbRI 85 residues - 9451 Da.

Residues 31-115 of the human TGF-B type I receptor

1

ALA

THR

ALA

LEU

GLN

CYS

PHE

CYS

HIS

LEU

2

CYS

THR

LYS

ASP

ASN

PHE

THR

CYS

VAL

THR

3

ASP

GLY

LEU

CYS

PHE

VAL

SER

VAL

THR

GLU

4

THR

ASP

LYS

VAL

ILE

HIS

ASN

SER

5

CYS

ILE

ALA

GLU

ILE

ASP

LEU

ILE

PRO

ARG

6

ASP

ARG

PRO

PHE

VAL

CYS

ALA

PRO

SER

7

LYS

THR

GLY

SER

VAL

THR

TYR

CYS

8

CYS

ASN

GLN

ASP

HIS

CYS

ASN

LYS

ILE

GLU

9

LEU

PRO

THR

VAL

Entity 2, TGM-D2 83 residues - 9296 Da.

The first two residues are part of a KpNI cut site

1

GLY

THR

ARG

CYS

SER

PRO

LEU

PRO

THR

ASN

2

ASP

THR

VAL

SER

PHE

GLU

TYR

LEU

LYS

ALA

3

THR

VAL

ASN

PRO

GLY

ILE

PHE

ASN

ILE

4

THR

VAL

HIS

PRO

ASP

ALA

SER

GLY

LYS

TYR

5

PRO

GLU

LEU

THR

TYR

ILE

LYS

ARG

ILE

CYS

6

LYS

ASN

PHE

PRO

THR

ASP

SER

ASN

VAL

GLN

7

GLY

HIS

ILE

GLY

MET

CYS

TYR

ASN

ALA

8

GLU

TRP

GLN

PHE

SER

THR

PRO

THR

CYS

9

PRO

ALA

SER

Samples:

sample_1: Transforming Growth Factor Beta Receptor 1 (TbRI), [U-98% 15N; U-95% 13C], 250 uM; TGM-1 D2 325 uM; D2O 5%; Na2HPO4 25 mM; NaCl 50 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4 - chemical shift assignment, chemical shift calculation

NMRFAM-SPARKY v1.2 - chemical shift assignment, chemical shift calculation

TOPSPIN v3.1 - collection

NMRPipe v2.6 - data analysis

PINE vI-PINE - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE II 800 MHz