BMRB Entry 51076

Name: Methyl side-chain chemical shift assignments for ribosomal protein bL12
Published: 2021-09-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51076

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Methyl side-chain chemical shift assignments for ribosomal protein bL12

Deposition date:

2021-09-06

Original release date:

2021-09-12

Authors:

Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John

Citation:

Citation: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John. "Nascent chain dynamics and ribosome interactions within folded ribosome-nascent chain complexes observed by NMR spectroscopy" Chem. Sci. 12, 13120-13126 (2021).
PubMed: 34745542

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSITKDQIIEAVAAMSVMDV VELISAMEEKFGVSAAAAVA VAAGPVEAAEEKTEFDVILK AAGANKVAVIKAVRGATGLG LKEAKDLVESAPAALKEGVS KDDAEALKKALEEAGAEVEV K

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	14
1H chemical shifts	42

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	bL12	1

Entities:

Entity 1, bL12 121 residues - Formula weight is not available

E.coli ribosomal protein bL12

1

MET

SER

ILE

THR

LYS

ASP

GLN

ILE

GLU

2

ALA

VAL

ALA

MET

SER

VAL

MET

ASP

VAL

3

VAL

GLU

LEU

ILE

SER

ALA

MET

GLU

LYS

4

PHE

GLY

VAL

SER

ALA

VAL

ALA

5

VAL

ALA

GLY

PRO

VAL

GLU

ALA

GLU

6

GLU

LYS

THR

GLU

PHE

ASP

VAL

ILE

LEU

LYS

7

ALA

GLY

ALA

ASN

LYS

VAL

ALA

VAL

ILE

8

LYS

ALA

VAL

ARG

GLY

ALA

THR

GLY

LEU

GLY

9

LEU

LYS

GLU

ALA

LYS

ASP

LEU

VAL

GLU

SER

10

ALA

PRO

ALA

LEU

LYS

GLU

GLY

VAL

SER

11

LYS

ASP

ALA

GLU

ALA

LEU

LYS

ALA

12

LEU

GLU

ALA

GLY

ALA

GLU

VAL

GLU

VAL

13

LYS

Samples:

sample_1: bL12, [U-13C; U-15N], 300 uM; HEPES 10 mM; magnesium chloride 12 mM; ammonium chloride 30 mM; BME 2 mM; DSS 0.001%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
4D HCccoNH TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

MATLAB - chemical shift assignment

NMRFAM-SPARKY v1.414 - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

SMILE - processing

NMR spectrometers:

Bruker AVANCE III 700 MHz

UNP	P0A7K2
AlphaFold	Q2M8S2