BMRB Entry 51075

Name: FLN5 filamin domain side-chain chemical shift assignments
Published: 2021-09-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51075

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FLN5 filamin domain side-chain chemical shift assignments

Deposition date:

2021-09-06

Original release date:

2021-09-12

Authors:

Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John

Citation:

Citation: Burridge, Charles; Waudby, Christopher; Wlodarski, Tomasz; Cassaignau, AnaIs; Cabrita, Lisa; Christodoulou, John. "Nascent chain dynamics and ribosome interactions within folded ribosome-nascent chain complexes observed by NMR spectroscopy" Chem. Sci. 12, 13120-13126 (2021).
PubMed: 34745542

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLDC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHASKPAPSAEHSYA EGEGLVKVFDNAPAEFTIFA VDTKGVARTDGGDPFEVAIN GPDGLVVDAKVTDNNDGTYG VVYDAPVEGNYNVNVTLRGN PIKNMPIDVKCIEG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	258
15N chemical shifts	96
1H chemical shifts	538

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FLN5	1

Entities:

Entity 1, FLN5 114 residues - Formula weight is not available

Fifth Domain from filamin. First 9 residues are non-native affinity tag.

1

MET

HIS

ALA

SER

LYS

2

PRO

ALA

PRO

SER

ALA

GLU

HIS

SER

TYR

ALA

3

GLU

GLY

GLU

GLY

LEU

VAL

LYS

VAL

PHE

ASP

4

ASN

ALA

PRO

ALA

GLU

PHE

THR

ILE

PHE

ALA

5

VAL

ASP

THR

LYS

GLY

VAL

ALA

ARG

THR

ASP

6

GLY

ASP

PRO

PHE

GLU

VAL

ALA

ILE

ASN

7

GLY

PRO

ASP

GLY

LEU

VAL

ASP

ALA

LYS

8

VAL

THR

ASP

ASN

ASP

GLY

THR

TYR

GLY

9

VAL

TYR

ASP

ALA

PRO

VAL

GLU

GLY

ASN

10

TYR

ASN

VAL

ASN

VAL

THR

LEU

ARG

GLY

ASN

11

PRO

ILE

LYS

ASN

MET

PRO

ILE

ASP

VAL

LYS

12

CYS

ILE

GLU

GLY

Samples:

sample_1: FLN5, [U-13C; U-15N], 500 uM; HEPES 10 mM; ammonium chloride 30 mM; magnesium chloride 12 mM; EDTA 1 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)C(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY v1.414 - chemical shift assignment

NMRPipe - processing

TOPSPIN - collection

SMILE - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz
Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks