BMRB Entry 5107
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5107
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Smith, Catherine; Wohnert, Jens; Sauer, Robert; Schwalbe, Harald. "Letter to the Editor: Assignments of the 1H, 13C, and 15N resonances of the
substrate-binding SSD domain from Lon protease" J. Biomol. NMR 21, 387-388 (2001).
PubMed: 11824761
Assembly members:
Lon (La) protease SSD, polymer, 101 residues, 11710 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLysS
Entity Sequences (FASTA):
Lon (La) protease SSD: MRLSGYTEDEKLNIAKRHLL
PKQIERNALKKGELTVDDSA
IIGIIRYYTREAGVRGLERE
ISKLCRKAVKQLLLDKSLKH
IEINGDNLHDYLGVQRFDYG
R
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 661 |
| 13C chemical shifts | 421 |
| 15N chemical shifts | 98 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lon SSD | 1 |
Entities:
Entity 1, Lon SSD 101 residues - 11710 Da.
| 1 | MET | ARG | LEU | SER | GLY | TYR | THR | GLU | ASP | GLU | ||||
| 2 | LYS | LEU | ASN | ILE | ALA | LYS | ARG | HIS | LEU | LEU | ||||
| 3 | PRO | LYS | GLN | ILE | GLU | ARG | ASN | ALA | LEU | LYS | ||||
| 4 | LYS | GLY | GLU | LEU | THR | VAL | ASP | ASP | SER | ALA | ||||
| 5 | ILE | ILE | GLY | ILE | ILE | ARG | TYR | TYR | THR | ARG | ||||
| 6 | GLU | ALA | GLY | VAL | ARG | GLY | LEU | GLU | ARG | GLU | ||||
| 7 | ILE | SER | LYS | LEU | CYS | ARG | LYS | ALA | VAL | LYS | ||||
| 8 | GLN | LEU | LEU | LEU | ASP | LYS | SER | LEU | LYS | HIS | ||||
| 9 | ILE | GLU | ILE | ASN | GLY | ASP | ASN | LEU | HIS | ASP | ||||
| 10 | TYR | LEU | GLY | VAL | GLN | ARG | PHE | ASP | TYR | GLY | ||||
| 11 | ARG |
Samples:
sample_1: Lon (La) protease SSD, [U-15N], 3.0 mM
sample_2: Lon (La) protease SSD, [U-15N; U-13C], 3.0 mM
cond_1: pH*: 5.3; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HCC(CO)NH | not available | not available | not available |
| CC(CO)NH | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
Software:
NMRpipe v1.8 - processing spectra
nmrView v1.8 - analyzing spectra
NMR spectrometers:
- Varian UnityInova 750 MHz
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB33916 BAE76219 BAG75989 BAH62055 BAI23813 |
| EMBL | CAD08909 CAP74973 CAQ30912 CAQ90073 CAQ97315 |
| GB | AAA16837 AAA24079 AAB40195 AAC36871 AAC73542 |
| PIR | AE0558 |
| PRF | 2004285A |
| REF | NP_286181 NP_308520 NP_414973 NP_455047 NP_459446 |
| SP | P0A9M0 P0A9M1 Q32JJ5 |
| AlphaFold | P0A9M0 P0A9M1 Q32JJ5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks